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Literature summary for 3.2.1.B26 extracted from

  • Grogan, D.W.
    Evidence that beta-galactosidase of Sulfolobus solfataricus is only one of several activities of a thermostable beta-D-glycosidase (1991), Appl. Environ. Microbiol., 57, 1644-1649.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
butanol the liberation of aglycone from aryl beta-D-glucosides is stimulated by alcohols in a manner suggesting specific interaction between alcohol and enzyme Saccharolobus solfataricus
ethanol the liberation of aglycone from aryl beta-D-glucosides is stimulated by alcohols in a manner suggesting specific interaction between alcohol and enzyme Saccharolobus solfataricus
methanol the liberation of aglycone from aryl beta-D-glucosides is stimulated by alcohols in a manner suggesting specific interaction between alcohol and enzyme Saccharolobus solfataricus
Propanol the liberation of aglycone from aryl beta-D-glucosides is stimulated by alcohols in a manner suggesting specific interaction between alcohol and enzyme Saccharolobus solfataricus

Inhibitors

Inhibitors Comment Organism Structure
1,4-D-galactonolactone 0.1 M, 98% inhibition of beta-galactoside hydrolysis, 90% inhibition of beta-glucoside hydrolysis Saccharolobus solfataricus
1,5-D-gluconolactone 0.1 M, complete inhibition of beta-galactoside hydrolysis and beta-glucoside hydrolysis Saccharolobus solfataricus
D-arabinose 0.1 M, 64% inhibition of beta-galactoside hydrolysis, 20% inhibition of beta-glucoside hydrolysis Saccharolobus solfataricus
D-cellobiose 0.1 M, 79% inhibition of beta-galactoside hydrolysis, 25% inhibition of beta-glucoside hydrolysis Saccharolobus solfataricus
D-fucose 0.1 M, 58% inhibition of beta-galactoside hydrolysis, 23% inhibition of beta-glucoside hydrolysis Saccharolobus solfataricus
D-galactose 0.1 M, 21% inhibition of beta-galactoside hydrolysis, slight activation of beta-glucoside hydrolysis Saccharolobus solfataricus
D-glucose 0.1 M, 37% inhibition of beta-galactoside hydrolysis, slight activation of beta-glucoside hydrolysis Saccharolobus solfataricus
lactose 0.1 M, 41% inhibition of beta-galactoside hydrolysis, 18% inhibition of beta-glucoside hydrolysis Saccharolobus solfataricus
Salicin 0.1 M, 97% inhibition of beta-galactoside hydrolysis, 92% inhibition of beta-glucoside hydrolysis Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.095
-
4-nitrophenyl beta-D-glucopyranoside pH 4.9, 77°C Saccharolobus solfataricus
0.13
-
4-nitrophenyl beta-D-fucopyranoside pH 4.9, 77°C Saccharolobus solfataricus
0.77
-
4-nitrophenyl beta-D-galactopyranoside pH 4.9, 77°C Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000 65000 gel filtration Saccharolobus solfataricus
70000
-
x * 70000, SDS-PAGE Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-
Saccharolobus solfataricus P2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-arabinopyranoside + H2O 14% of the activity compared to 4-nitrophenyl-beta-D-galactopyranoside Saccharolobus solfataricus 4-nitrophenol + alpha-L-fucopyranose
-
?
4-nitrophenyl alpha-L-arabinopyranoside + H2O 14% of the activity compared to 4-nitrophenyl-beta-D-galactopyranoside Saccharolobus solfataricus P2 4-nitrophenol + alpha-L-fucopyranose
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O 73% of the activity compared to 4-nitrophenyl-beta-D-galactopyranoside Saccharolobus solfataricus 4-nitrophenol + beta-D-fucopyranose
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O 73% of the activity compared to 4-nitrophenyl-beta-D-galactopyranoside Saccharolobus solfataricus P2 4-nitrophenol + beta-D-fucopyranose
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
-
Saccharolobus solfataricus 4-nitrophenol + beta-D-galactopyranose
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
-
Saccharolobus solfataricus P2 4-nitrophenol + beta-D-galactopyranose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O 52% of the activity compared to 4-nitrophenyl-beta-D-galactopyranoside Saccharolobus solfataricus 4-nitrophenol + D-glucopyranose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O 52% of the activity compared to 4-nitrophenyl-beta-D-galactopyranoside Saccharolobus solfataricus P2 4-nitrophenol + D-glucopyranose
-
?
additional information the enzyme hydrolyzes a variety of low-molecular-weight, beta-linked glycosides. Chromogenic beta-D-galactosides and beta-D-glucosides are hydrolyzed at a common active site. beta-Glucosides and beta-fucosides represent the preferred substrates Saccharolobus solfataricus ?
-
?
additional information the enzyme hydrolyzes a variety of low-molecular-weight, beta-linked glycosides. Chromogenic beta-D-galactosides and beta-D-glucosides are hydrolyzed at a common active site. beta-Glucosides and beta-fucosides represent the preferred substrates Saccharolobus solfataricus P2 ?
-
?

Subunits

Subunits Comment Organism
monomer x * 70000, SDS-PAGE Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
beta-D-glycosidase
-
Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
77
-
assay at Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.9
-
assay at Saccharolobus solfataricus