Activating Compound | Comment | Organism | Structure |
---|---|---|---|
butanol | the liberation of aglycone from aryl beta-D-glucosides is stimulated by alcohols in a manner suggesting specific interaction between alcohol and enzyme | Saccharolobus solfataricus | |
ethanol | the liberation of aglycone from aryl beta-D-glucosides is stimulated by alcohols in a manner suggesting specific interaction between alcohol and enzyme | Saccharolobus solfataricus | |
methanol | the liberation of aglycone from aryl beta-D-glucosides is stimulated by alcohols in a manner suggesting specific interaction between alcohol and enzyme | Saccharolobus solfataricus | |
Propanol | the liberation of aglycone from aryl beta-D-glucosides is stimulated by alcohols in a manner suggesting specific interaction between alcohol and enzyme | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,4-D-galactonolactone | 0.1 M, 98% inhibition of beta-galactoside hydrolysis, 90% inhibition of beta-glucoside hydrolysis | Saccharolobus solfataricus | |
1,5-D-gluconolactone | 0.1 M, complete inhibition of beta-galactoside hydrolysis and beta-glucoside hydrolysis | Saccharolobus solfataricus | |
D-arabinose | 0.1 M, 64% inhibition of beta-galactoside hydrolysis, 20% inhibition of beta-glucoside hydrolysis | Saccharolobus solfataricus | |
D-cellobiose | 0.1 M, 79% inhibition of beta-galactoside hydrolysis, 25% inhibition of beta-glucoside hydrolysis | Saccharolobus solfataricus | |
D-fucose | 0.1 M, 58% inhibition of beta-galactoside hydrolysis, 23% inhibition of beta-glucoside hydrolysis | Saccharolobus solfataricus | |
D-galactose | 0.1 M, 21% inhibition of beta-galactoside hydrolysis, slight activation of beta-glucoside hydrolysis | Saccharolobus solfataricus | |
D-glucose | 0.1 M, 37% inhibition of beta-galactoside hydrolysis, slight activation of beta-glucoside hydrolysis | Saccharolobus solfataricus | |
lactose | 0.1 M, 41% inhibition of beta-galactoside hydrolysis, 18% inhibition of beta-glucoside hydrolysis | Saccharolobus solfataricus | |
Salicin | 0.1 M, 97% inhibition of beta-galactoside hydrolysis, 92% inhibition of beta-glucoside hydrolysis | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.095 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 4.9, 77°C | Saccharolobus solfataricus | |
0.13 | - |
4-nitrophenyl beta-D-fucopyranoside | pH 4.9, 77°C | Saccharolobus solfataricus | |
0.77 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 4.9, 77°C | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | 65000 | gel filtration | Saccharolobus solfataricus |
70000 | - |
x * 70000, SDS-PAGE | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Saccharolobus solfataricus P2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-L-arabinopyranoside + H2O | 14% of the activity compared to 4-nitrophenyl-beta-D-galactopyranoside | Saccharolobus solfataricus | 4-nitrophenol + alpha-L-fucopyranose | - |
? | |
4-nitrophenyl alpha-L-arabinopyranoside + H2O | 14% of the activity compared to 4-nitrophenyl-beta-D-galactopyranoside | Saccharolobus solfataricus P2 | 4-nitrophenol + alpha-L-fucopyranose | - |
? | |
4-nitrophenyl beta-D-fucopyranoside + H2O | 73% of the activity compared to 4-nitrophenyl-beta-D-galactopyranoside | Saccharolobus solfataricus | 4-nitrophenol + beta-D-fucopyranose | - |
? | |
4-nitrophenyl beta-D-fucopyranoside + H2O | 73% of the activity compared to 4-nitrophenyl-beta-D-galactopyranoside | Saccharolobus solfataricus P2 | 4-nitrophenol + beta-D-fucopyranose | - |
? | |
4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + beta-D-galactopyranose | - |
? | |
4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Saccharolobus solfataricus P2 | 4-nitrophenol + beta-D-galactopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | 52% of the activity compared to 4-nitrophenyl-beta-D-galactopyranoside | Saccharolobus solfataricus | 4-nitrophenol + D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | 52% of the activity compared to 4-nitrophenyl-beta-D-galactopyranoside | Saccharolobus solfataricus P2 | 4-nitrophenol + D-glucopyranose | - |
? | |
additional information | the enzyme hydrolyzes a variety of low-molecular-weight, beta-linked glycosides. Chromogenic beta-D-galactosides and beta-D-glucosides are hydrolyzed at a common active site. beta-Glucosides and beta-fucosides represent the preferred substrates | Saccharolobus solfataricus | ? | - |
? | |
additional information | the enzyme hydrolyzes a variety of low-molecular-weight, beta-linked glycosides. Chromogenic beta-D-galactosides and beta-D-glucosides are hydrolyzed at a common active site. beta-Glucosides and beta-fucosides represent the preferred substrates | Saccharolobus solfataricus P2 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | x * 70000, SDS-PAGE | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
beta-D-glycosidase | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
77 | - |
assay at | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.9 | - |
assay at | Saccharolobus solfataricus |