Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | about 90% residual activity at 2 mM | Pseudoalteromonas sp. NJ21 | |
Cd2+ | about 85% residual activity at 2 mM | Pseudoalteromonas sp. NJ21 | |
Cu2+ | about 40% residual activity at 2 mM | Pseudoalteromonas sp. NJ21 | |
EDTA | about 22% residual activity at 2 mM | Pseudoalteromonas sp. NJ21 | |
Fe2+ | about 68% residual activity at 2 mM | Pseudoalteromonas sp. NJ21 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Pseudoalteromonas sp. NJ21 | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | about 155% activity at 2 mM | Pseudoalteromonas sp. NJ21 | |
Mg2+ | about 118% activity at 2 mM | Pseudoalteromonas sp. NJ21 | |
Mn2+ | about 122% activity at 2 mM | Pseudoalteromonas sp. NJ21 | |
additional information | not influenced by Sr2+ and Co2+ | Pseudoalteromonas sp. NJ21 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudoalteromonas sp. NJ21 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Q-Sepharose column chromatography, and Sephacryl S-200 gel filtration | Pseudoalteromonas sp. NJ21 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
agar + H2O | - |
Pseudoalteromonas sp. NJ21 | neoagarobiose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 80000, SDS-PAGE | Pseudoalteromonas sp. NJ21 |
Synonyms | Comment | Organism |
---|---|---|
Aga21 | - |
Pseudoalteromonas sp. NJ21 |
extracellular beta-agarase | - |
Pseudoalteromonas sp. NJ21 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Pseudoalteromonas sp. NJ21 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 30 | the enzyme maintains 85% of the maximum activity at 10°C | Pseudoalteromonas sp. NJ21 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 50 | up to 80% thermostability of the enzyme is retained at 10°C and 30 °C for 180 min. When the temperature is increased to 50°C for 30 min, the enzyme relative activity is decreased to 10-20%. Nevertheless, the enzyme is fairly stable (almost 70% relative activity) at 40°C for 30 min | Pseudoalteromonas sp. NJ21 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pseudoalteromonas sp. NJ21 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | the enzyme is stable (more than 70% relative activity) in buffers with a pH range of 6.5-8.5 | Pseudoalteromonas sp. NJ21 |