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Literature summary for 3.2.1.B1 extracted from
- Purification and characterization of agarase from Rhodococcus sp. Q5, a novel agarolytic bacterium isolated from printing and dyeing wastewater (2013), Aquaculture, 372-375, 74-79 .
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 15% residual activity at 1 mM | Rhodococcus sp. Q5 |  |
| Ba2+ | 16% residual activity at 1 mM | Rhodococcus sp. Q5 | |
| EDTA | 77% residual activity at 1 mM | Rhodococcus sp. Q5 | |
| Fe2+ | 53% residual activity at 1 mM | Rhodococcus sp. Q5 | |
| Fe3+ | 15% residual activity at 1 mM | Rhodococcus sp. Q5 | |
| Mg2+ | 46% residual activity at 1 mM | Rhodococcus sp. Q5 | |
| Pb2+ | 7% residual activity at 1 mM | Rhodococcus sp. Q5 | |
| SDS | 54% residual activity at 1 mM | Rhodococcus sp. Q5 | |
| Sn2+ | 30% residual activity at 1 mM | Rhodococcus sp. Q5 | |
| Zn2+ | 23% residual activity at 1 mM | Rhodococcus sp. Q5 | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Rhodococcus sp. Q5 | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no significant activation or inhibition of the enzyme is observed in the presence of Na+, K+ or Ca2+ | Rhodococcus sp. Q5 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agar + H2O | Rhodococcus sp. Q5 | - |
neoagarotriose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodococcus sp. Q5 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial purification by ammonium sulfate precipitation | Rhodococcus sp. Q5 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agar + H2O | - |
Rhodococcus sp. Q5 | neoagarotriose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 54000, SDS-PAGE | Rhodococcus sp. Q5 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Rhodococcus sp. Q5 |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | about 45% activity 20°C, about 70% activity 30°C, 100% activity 40°C, about 45% activity 50°C | Rhodococcus sp. Q5 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 40 | more than 90% activity is retained after 30 min at 10-40°C | Rhodococcus sp. Q5 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Rhodococcus sp. Q5 |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7 | more than 50% activity between pH 5.0 and 7.0 | Rhodococcus sp. Q5 |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | the enzyme is stable for 30 min in the pH range from pH 5.0 to 8.0, and more than 87% of the residual activity is retained | Rhodococcus sp. Q5 |
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