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Literature summary for 3.2.1.B1 extracted from
- Purification and characterization of agarase from marine bacteria Acinetobacter sp. PS12B and its use for preparing bioactive hydrolysate from agarophyte red seaweed Gracilaria verrucosa (2018), Appl. Biochem. Biotechnol., 186, 66-84 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | about 135% activity at 10 mM | Acinetobacter junii |  |
| dithiothreitol | about 145% activity at 10 mM | Acinetobacter junii | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetone | 86.9% residual activity at 5% (v/v) | Acinetobacter junii | |
| DMSO | 79.3% residual activity at 5% (v/v) | Acinetobacter junii | |
| EDTA | EDTA reduces the agarase activity by 58% at 5 mM | Acinetobacter junii | |
| ethanol | 76.9% residual activity at 5% (v/v) | Acinetobacter junii | |
| ethyl acetate | 12.1% residual activity at 5% (v/v) | Acinetobacter junii | |
| hexane | 76.9% residual activity at 5% (v/v) | Acinetobacter junii | |
| methanol | 72.6% residual activity at 5% (v/v) | Acinetobacter junii | |
| SDS | about 83% residual activity at 5 mM | Acinetobacter junii | |
| Urea | about 80% residual activity at 5 mM | Acinetobacter junii | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Acinetobacter junii | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | 133.4% activity at 10 mM | Acinetobacter junii | |
| Mn2+ | 134.4% activity at 5 mM | Acinetobacter junii | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acinetobacter junii | - |
- |
- |
| Acinetobacter junii PS12B | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation and DEAE-cellulose column chromatography | Acinetobacter junii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agar + H2O | - |
Acinetobacter junii | neoagarotriose + neoagarohexaose + neoagaroheptaose | - |
? | |
| agar + H2O | - |
Acinetobacter junii PS12B | neoagarotriose + neoagarohexaose + neoagaroheptaose | - |
? | |
| seaweed + H2O | - |
Acinetobacter junii | ? | - |
? | |
| seaweed + H2O | - |
Acinetobacter junii PS12B | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 24000, SDS-PAGE | Acinetobacter junii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endo-acting beta-agarase | - |
Acinetobacter junii |
| endo-type beta-agarase | - |
Acinetobacter junii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Acinetobacter junii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
the enzyme is stable up to 1 h at 50°C | Acinetobacter junii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Acinetobacter junii |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | the purified enzyme is most stable at pH 7.0-8.0 with more than 98% residual activity after 24 h. The residual activity of enzyme reduces to less than 50% when incubated for 24 h at pH 4.0 | Acinetobacter junii |
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