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Literature summary for 3.2.1.B1 extracted from

  • Su, Q.; Jin, T.; Yu, Y.; Yang, M.; Mou, H.; Li, L.
    Extracellular expression of a novel beta-agarase from Microbulbifer sp. Q7, isolated from the gut of sea cucumber (2017), AMB Express, 7, 220 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 141.05% activity at 0.5% (v/v) Microbulbifer sp. Q7
dithiothreitol 103.44% activity at 5 mM Microbulbifer sp. Q7
additional information not influenced by Triton X-100 Microbulbifer sp. Q7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Microbulbifer sp. Q7

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 96.78% residual activity at 5 mM Microbulbifer sp. Q7
Cu2+ 6.2% residual activity at 5 mM Microbulbifer sp. Q7
EDTA 86.47% residual activity at 5 mM Microbulbifer sp. Q7
Fe3+ 34.65% residual activity at 5 mM Microbulbifer sp. Q7
K+ 97.26% residual activity at 5 mM Microbulbifer sp. Q7
Li+ 92% residual activity at 5 mM Microbulbifer sp. Q7
Mg2+ 76.92% residual activity at 5 mM Microbulbifer sp. Q7
additional information not inhibited by urea and SDS Microbulbifer sp. Q7
Tween-80 89.72% residual activity at 0.5% (v/v) Microbulbifer sp. Q7
Zn2+ 14.97% residual activity at 5 mM Microbulbifer sp. Q7

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Microbulbifer sp. Q7
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Na+ 116.46% activity at 5 mM Microbulbifer sp. Q7

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
agarose + H2O Microbulbifer sp. Q7
-
neoagarohexaose + neoagarotetraose + D-galactose
-
?

Organism

Organism UniProt Comment Textmining
Microbulbifer sp. Q7
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni Sepharose column chromatography Microbulbifer sp. Q7

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agarose + H2O
-
Microbulbifer sp. Q7 neoagarohexaose + neoagarotetraose + D-galactose
-
?

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE Microbulbifer sp. Q7

Synonyms

Synonyms Comment Organism
AgaB
-
Microbulbifer sp. Q7
beta-agarase
-
Microbulbifer sp. Q7

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Microbulbifer sp. Q7

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 50 more than 50% activity between 35 and 50°C Microbulbifer sp. Q7

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37 45 the enzyme retains 90.12% activity at 37°C after 2 h, more than 55% activity at 40 and 42°C after 1 h, and 36.16% activity at 45°C after 1 h Microbulbifer sp. Q7

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Microbulbifer sp. Q7

pH Range

pH Minimum pH Maximum Comment Organism
4 10 more than 70% activity between pH 4.0 and 10.0 Microbulbifer sp. Q7

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 the enzyme is stable between pH 6.0 and pH 9.0 for 1 h Microbulbifer sp. Q7