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Literature summary for 3.2.1.B1 extracted from

  • Cui, F.; Dong, S.; Shi, X.; Zhao, X.; Zhang, X.H.
    Overexpression and characterization of a novel thermostable beta-agarase YM01-3, from marine bacterium Catenovulum agarivorans YM01(T) (2014), Mar. Drugs, 12, 2731-2747.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Catenovulum agarivorans

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 34% residual activity at 1 mM Catenovulum agarivorans
EDTA 81% residual activity at 1 mM Catenovulum agarivorans
Fe3+ 14% residual activity at 1 mM Catenovulum agarivorans
Mg2+ 88% residual activity at 1 mM Catenovulum agarivorans
Mn2+ 28% residual activity at 1 mM Catenovulum agarivorans
additional information urea and SDS do not have obvious effects on the activity Catenovulum agarivorans
Ni2+ 1% residual activity at 10 mM Catenovulum agarivorans

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Catenovulum agarivorans
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 113% activity at 1 mM Catenovulum agarivorans
K+ 122% activity at 1 mM Catenovulum agarivorans
Na+ 122% activity at 1 mM Catenovulum agarivorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
x * 40000, SDS-PAGE Catenovulum agarivorans
46900
-
x * 46900, calculated from amino acid sequence Catenovulum agarivorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
agarose + H2O Catenovulum agarivorans
-
neoagarotetraose + neoagarohexaose + neoagarooctaose + neoagarodecaose neoagarotetraose and neoagarohexaose are the main products ?
agarose + H2O Catenovulum agarivorans YM01T
-
neoagarotetraose + neoagarohexaose + neoagarooctaose + neoagarodecaose neoagarotetraose and neoagarohexaose are the main products ?

Organism

Organism UniProt Comment Textmining
Catenovulum agarivorans T1WFD0
-
-
Catenovulum agarivorans YM01T T1WFD0
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Catenovulum agarivorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agarose + H2O
-
Catenovulum agarivorans neoagarotetraose + neoagarohexaose + neoagarooctaose + neoagarodecaose neoagarotetraose and neoagarohexaose are the main products ?
agarose + H2O
-
Catenovulum agarivorans YM01T neoagarotetraose + neoagarohexaose + neoagarooctaose + neoagarodecaose neoagarotetraose and neoagarohexaose are the main products ?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Catenovulum agarivorans
? x * 46900, calculated from amino acid sequence Catenovulum agarivorans

Synonyms

Synonyms Comment Organism
beta-agarase
-
Catenovulum agarivorans
YM01-3
-
Catenovulum agarivorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Catenovulum agarivorans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 80 the enzyme retains more than 80% agarolytic activity after being kept in 50°C for 1 h, 13% residual activity after incubation in 80°C for 1 h, and 12% residual activity if boiled for 5 min Catenovulum agarivorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Catenovulum agarivorans

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 9 the enzyme retains more than 80% of activity after incubation at a wide range of 4.0–9.0 for 12 h at 4°C Catenovulum agarivorans