Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Catenovulum agarivorans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 34% residual activity at 1 mM | Catenovulum agarivorans | |
EDTA | 81% residual activity at 1 mM | Catenovulum agarivorans | |
Fe3+ | 14% residual activity at 1 mM | Catenovulum agarivorans | |
Mg2+ | 88% residual activity at 1 mM | Catenovulum agarivorans | |
Mn2+ | 28% residual activity at 1 mM | Catenovulum agarivorans | |
additional information | urea and SDS do not have obvious effects on the activity | Catenovulum agarivorans | |
Ni2+ | 1% residual activity at 10 mM | Catenovulum agarivorans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Catenovulum agarivorans | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 113% activity at 1 mM | Catenovulum agarivorans | |
K+ | 122% activity at 1 mM | Catenovulum agarivorans | |
Na+ | 122% activity at 1 mM | Catenovulum agarivorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, SDS-PAGE | Catenovulum agarivorans |
46900 | - |
x * 46900, calculated from amino acid sequence | Catenovulum agarivorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
agarose + H2O | Catenovulum agarivorans | - |
neoagarotetraose + neoagarohexaose + neoagarooctaose + neoagarodecaose | neoagarotetraose and neoagarohexaose are the main products | ? | |
agarose + H2O | Catenovulum agarivorans YM01T | - |
neoagarotetraose + neoagarohexaose + neoagarooctaose + neoagarodecaose | neoagarotetraose and neoagarohexaose are the main products | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Catenovulum agarivorans | T1WFD0 | - |
- |
Catenovulum agarivorans YM01T | T1WFD0 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Catenovulum agarivorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
agarose + H2O | - |
Catenovulum agarivorans | neoagarotetraose + neoagarohexaose + neoagarooctaose + neoagarodecaose | neoagarotetraose and neoagarohexaose are the main products | ? | |
agarose + H2O | - |
Catenovulum agarivorans YM01T | neoagarotetraose + neoagarohexaose + neoagarooctaose + neoagarodecaose | neoagarotetraose and neoagarohexaose are the main products | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE | Catenovulum agarivorans |
? | x * 46900, calculated from amino acid sequence | Catenovulum agarivorans |
Synonyms | Comment | Organism |
---|---|---|
beta-agarase | - |
Catenovulum agarivorans |
YM01-3 | - |
Catenovulum agarivorans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Catenovulum agarivorans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 80 | the enzyme retains more than 80% agarolytic activity after being kept in 50°C for 1 h, 13% residual activity after incubation in 80°C for 1 h, and 12% residual activity if boiled for 5 min | Catenovulum agarivorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Catenovulum agarivorans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | the enzyme retains more than 80% of activity after incubation at a wide range of 4.09.0 for 12 h at 4°C | Catenovulum agarivorans |