General Stability | Organism |
---|---|
the enzyme remains stable in the presence of up to 4 M NaCl | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetone | about 30% residual activity at 10% (v/v) | Pseudomonas sp. | |
Benzene | about 70% residual activity at 10% (v/v) | Pseudomonas sp. | |
butan-1-ol | about 30% residual activity at 10% (v/v) | Pseudomonas sp. | |
chloroform | about 25% residual activity at 10% (v/v) | Pseudomonas sp. | |
Co2+ | about 30% residual activity at 1 mM | Pseudomonas sp. | |
Cyclohexane | about 35% residual activity at 10% (v/v) | Pseudomonas sp. | |
Dichloromethane | about 20% residual activity at 10% (v/v) | Pseudomonas sp. | |
DMSO | about 40% residual activity at 10% (v/v) | Pseudomonas sp. | |
EDTA | about 20% residual activity at 1 mM | Pseudomonas sp. | |
ethenol | about 30% residual activity at 10% (v/v) | Pseudomonas sp. | |
heptane | about 50% residual activity at 10% (v/v) | Pseudomonas sp. | |
Hg2+ | about 80% residual activity at 1 mM | Pseudomonas sp. | |
Isopropanol | about 40% residual activity at 10% (v/v) | Pseudomonas sp. | |
Li+ | about 80% residual activity at 1 mM | Pseudomonas sp. | |
methanol | about 35% residual activity at 10% (v/v) | Pseudomonas sp. | |
Mg2+ | about 80% residual activity at 1 mM | Pseudomonas sp. | |
Pb2+ | about 50% residual activity at 1 mM | Pseudomonas sp. | |
phenylmethylsulfonyl fluoride | about 60% residual activity at 1 mM | Pseudomonas sp. | |
SDS | about 60% residual activity at 10% (w/v) | Pseudomonas sp. | |
Toluene | about 40% residual activity at 10% (v/v) | Pseudomonas sp. | |
Triton X-100 | 58% residual activity at 10% (v/v) | Pseudomonas sp. | |
Tween 20 | 89.2% residual activity at 10% (v/v) | Pseudomonas sp. | |
Tween 80 | 60.2% residual activity at 10% (v/v) | Pseudomonas sp. | |
Zn2+ | about 30% residual activity at 1 mM | Pseudomonas sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Pseudomonas sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | about 140% activity at 1 mM | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
x * 66000, SDS-PAGE | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
agar + H2O | Pseudomonas sp. | - |
neoagarobiose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE Sepharose column chromatography, and DEAE Sephadex A-50 gel filtration | Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
73.87 | - |
crude enzyme, at pH 9.0 and 35°C | Pseudomonas sp. |
615.5 | - |
after 8.33fold purification, at pH 9.0 and 35°C | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
agar + H2O | - |
Pseudomonas sp. | neoagarobiose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 66000, SDS-PAGE | Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
exo-beta-agarase | - |
Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
the enzyme loses 50% of its activity at 35°C after an incubation of 1 h | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Pseudomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
10 | 11 | the enzyme shows residual activities of about 85.2 and 78.2% at pH 10 and 11.0 | Pseudomonas sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
12 | 13 | the enzyme retains about 62% of its activity at pH 12.0 and almost 50% at pH 13.0 after a pre-incubation of 1 h | Pseudomonas sp. |