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Literature summary for 3.2.1.B1 extracted from

  • Feng, Z.; Li, M.
    Purification and characterization of agarase from Rhodococcus sp. Q5, a novel agarolytic bacterium isolated from printing and dyeing wastewater (2013), Aquaculture, 372-375, 74-79.
No PubMed abstract available
Search for more literature for 3.2.1.B1

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 15% residual activity at 1 mM Rhodococcus sp.
Ba2+ 16% residual activity at 1 mM Rhodococcus sp.
EDTA 77% residual activity at 1 mM Rhodococcus sp.
Fe2+ 53% residual activity at 1 mM Rhodococcus sp.
Fe3+ 15% residual activity at 1 mM Rhodococcus sp.
Mg2+ 46% residual activity at 1 mM Rhodococcus sp.
Pb2+ 7% residual activity at 1 mM Rhodococcus sp.
SDS 54% residual activity at 1 mM Rhodococcus sp.
Sn2+ 30% residual activity at 1 mM Rhodococcus sp.
Zn2+ 23% residual activity at 1 mM Rhodococcus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Rhodococcus sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no influence of the agarose in the presence of Na+, K+ or Ca2+ Rhodococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
 x * 54000, SDS-PAGE Rhodococcus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
agarose + H2O Rhodococcus sp.
-
 ? the enzyme mainly produces trisaccharide, as well as a small amount of disaccharides, tetrose, pentasaccharide, and hexose ?

Organism

Organism UniProt Comment Textmining
Rhodococcus sp.
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agarose + H2O
-
 Rhodococcus sp. ? the enzyme mainly produces trisaccharide, as well as a small amount of disaccharides, tetrose, pentasaccharide, and hexose ?

Subunits

Subunits Comment Organism
? x * 54000, SDS-PAGE Rhodococcus sp.

Synonyms

Synonyms Comment Organism
AgaA
-
 Rhodococcus sp.
beta-agarase
-
 Rhodococcus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Rhodococcus sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 60 about 30% activity at 10°C, about 40% activity at 20°C, about 70% activity at 30°C, 100% activity at 40°C, about 50% activity at 50°C, about 20% activity at 60°C Rhodococcus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10 40 the activity of agarase is stable at a low temperature and retains more than 90% of its activity up to a temperature of 40°C, after 30 min incubation Rhodococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Rhodococcus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8 the enzyme is stable in the pH range from pH 5.0 to 8.0 with more than 87% of the residual activity retained after 30 min Rhodococcus sp.