Activating Compound | Comment | Organism | Structure |
---|---|---|---|
beta-mercaptoethanol | 123% activity at 10 mM | Agarivorans sp. | |
DL-dithiothreitol | 121% activity at 10 mM | Agarivorans sp. |
Cloned (Comment) | Organism |
---|---|
the mature agarase is highly expressed extracellularly in Escherichia coli BL21 (DE3) cells | Agarivorans sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis-(2-nitrobenzoic acid) | 69% residual activity at 1 mM | Agarivorans sp. | |
Cu2+ | 69% residual activity at 1 mM | Agarivorans sp. | |
EDTA | 69% residual activity at 10 mM | Agarivorans sp. | |
Mg2+ | 65% residual activity at 1 mM | Agarivorans sp. | |
additional information | no significant activation or inhibition is observed urea | Agarivorans sp. | |
SDS | 53% residual activity at 10 mM | Agarivorans sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | 116% activity at 1 mM | Agarivorans sp. | |
additional information | no significant activation or inhibition is observed by metal ions Na+, K+, Ca2+, and Fe3+ | Agarivorans sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47200 | - |
x * 47200, SDS-PAGE | Agarivorans sp. |
51200 | - |
x * 51200, calculated from amino acid sequence | Agarivorans sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agarivorans sp. | C9WIW7 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Agarivorans sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
agarose + H2O | the recombinant enzyme hydrolyzes the beta-1,4-glycosidic linkages of agarose | Agarivorans sp. | neoagarotetraose + neoagarohexaose | main products | ? | |
additional information | the enzyme cannot cleave neoagarohexaose and shorter oligomers | Agarivorans sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 47200, SDS-PAGE | Agarivorans sp. |
? | x * 51200, calculated from amino acid sequence | Agarivorans sp. |
Synonyms | Comment | Organism |
---|---|---|
AgaA | - |
Agarivorans sp. |
beta-agarase | - |
Agarivorans sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Agarivorans sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 80 | 100% activity between 20 and40°C, about 50% activity at 50°C, about 10% activity between 60-80°C (after incubation for 1 h) | Agarivorans sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Agarivorans sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 11 | the enzyme still retains more than 95% activity after incubation at pH 3.0-11.0 for 1 h | Agarivorans sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Agarivorans sp. | calculated from amino acid sequence | - |
4.6 |