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Literature summary for 3.2.1.B1 extracted from

  • Long, M.; Yu, Z.; Xu, X.
    A novel beta-agarase with high pH stability from marine Agarivorans sp. LQ48 (2010), Mar. Biotechnol., 12, 62-69.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
beta-mercaptoethanol 123% activity at 10 mM Agarivorans sp.
DL-dithiothreitol 121% activity at 10 mM Agarivorans sp.

Cloned(Commentary)

Cloned (Comment) Organism
the mature agarase is highly expressed extracellularly in Escherichia coli BL21 (DE3) cells Agarivorans sp.

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis-(2-nitrobenzoic acid) 69% residual activity at 1 mM Agarivorans sp.
Cu2+ 69% residual activity at 1 mM Agarivorans sp.
EDTA 69% residual activity at 10 mM Agarivorans sp.
Mg2+ 65% residual activity at 1 mM Agarivorans sp.
additional information no significant activation or inhibition is observed urea Agarivorans sp.
SDS 53% residual activity at 10 mM Agarivorans sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 116% activity at 1 mM Agarivorans sp.
additional information no significant activation or inhibition is observed by metal ions Na+, K+, Ca2+, and Fe3+ Agarivorans sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47200
-
x * 47200, SDS-PAGE Agarivorans sp.
51200
-
x * 51200, calculated from amino acid sequence Agarivorans sp.

Organism

Organism UniProt Comment Textmining
Agarivorans sp. C9WIW7
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Agarivorans sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agarose + H2O the recombinant enzyme hydrolyzes the beta-1,4-glycosidic linkages of agarose Agarivorans sp. neoagarotetraose + neoagarohexaose main products ?
additional information the enzyme cannot cleave neoagarohexaose and shorter oligomers Agarivorans sp. ?
-
?

Subunits

Subunits Comment Organism
? x * 47200, SDS-PAGE Agarivorans sp.
? x * 51200, calculated from amino acid sequence Agarivorans sp.

Synonyms

Synonyms Comment Organism
AgaA
-
Agarivorans sp.
beta-agarase
-
Agarivorans sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Agarivorans sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 80 100% activity between 20 and40°C, about 50% activity at 50°C, about 10% activity between 60-80°C (after incubation for 1 h) Agarivorans sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Agarivorans sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 11 the enzyme still retains more than 95% activity after incubation at pH 3.0-11.0 for 1 h Agarivorans sp.

pI Value

Organism Comment pI Value Maximum pI Value
Agarivorans sp. calculated from amino acid sequence
-
4.6