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Literature summary for 3.2.1.99 extracted from

  • Park, J.M.; Jang, M.U.; Kang, J.H.; Kim, M.J.; Lee, S.W.; Song, Y.B.; Shin, C.S.; Han, N.S.; Kim, T.J.
    Detailed modes of action and biochemical characterization of endo-arabinanase from Bacillus licheniformis DSM13 (2012), J. Microbiol., 50, 1041-1046.
    View publication on PubMed
Search for more literature for 3.2.1.99

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bacillus licheniformis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 x * 30000, recombinant enzyme, SDS-PAGE Bacillus licheniformis
36358
-
 x * 36358, calculated from amino acid sequence Bacillus licheniformis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1,5-alpha-L-arabinan + H2O Bacillus licheniformis
-
 oligo-alpha-(1->5)-L-arabinoside
-
 ?
1,5-alpha-L-arabinan + H2O Bacillus licheniformis DSM 13
-
 oligo-alpha-(1->5)-L-arabinoside
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus licheniformis Q65GB9
-
-
Bacillus licheniformis Q65L63
-
-
Bacillus licheniformis DSM 13 Q65GB9
-
-
Bacillus licheniformis DSM 13 Q65L63
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Bacillus licheniformis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.13
-
 sugar beet arabinan as substrate, in 50 mM sodium acetate buffer (pH 6.0) at 55°C Bacillus licheniformis
64.52
-
 with linear 1,5-alpha-L-arabinan as substrate, in 50 mM sodium acetate buffer (pH 6.0) at 55°C Bacillus licheniformis
71.69
-
 with debranched 1,5-alpha-L-arabinan as substrate, in 50 mM sodium acetate buffer (pH 6.0) at 55°C Bacillus licheniformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,5-alpha-L-arabinan + H2O
-
 Bacillus licheniformis oligo-alpha-(1->5)-L-arabinoside
-
 ?
1,5-alpha-L-arabinan + H2O
-
 Bacillus licheniformis DSM 13 oligo-alpha-(1->5)-L-arabinoside
-
 ?
debranched 1,5-alpha-L-arabinan + H2O highest activity Bacillus licheniformis ?
-
 ?
debranched 1,5-alpha-L-arabinan + H2O highest activity Bacillus licheniformis DSM 13 ?
-
 ?
linear 1,5-alpha-L-arabinan + H2O
-
 Bacillus licheniformis ?
-
 ?
linear 1,5-alpha-L-arabinan + H2O
-
 Bacillus licheniformis DSM 13 ?
-
 ?
additional information the enzyme randomly cleaves the internal alpha-(1,5)-linked L-arabinofuranosyl residues of a branchless arabinan backbone to release arabinotriose mainly, although a small amount of arabino-oligosaccharide intermediates is also liberated Bacillus licheniformis ?
-
 ?
additional information the enzyme randomly cleaves the internal alpha-(1,5)-linked L-arabinofuranosyl residues of a branchless arabinan backbone to release arabinotriose mainly, although a small amount of arabino-oligosaccharide intermediates is also liberated Bacillus licheniformis DSM 13 ?
-
 ?
sugar beet arabinan + H2O weak activity Bacillus licheniformis ?
-
 ?
sugar beet arabinan + H2O weak activity Bacillus licheniformis DSM 13 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 30000, recombinant enzyme, SDS-PAGE Bacillus licheniformis
? x * 36358, calculated from amino acid sequence Bacillus licheniformis

Synonyms

Synonyms Comment Organism
abnA1 isoform Bacillus licheniformis
abnA2 isoform Bacillus licheniformis
ABNase
-
 Bacillus licheniformis
endo-(1,5)-alpha-L-arabinanase
-
 Bacillus licheniformis
endo-arabinanase
-
 Bacillus licheniformis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Bacillus licheniformis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 about 45% activity at 30°C, about 65% activity at 40°C, about 85% activity at 50°C, about 80% activity at 60°C, and about 20% activity at 70°C Bacillus licheniformis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 60 the half-life of the enzyme at 50, 55, and 60°C are 280, 152, and 30 min, respectively Bacillus licheniformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Bacillus licheniformis

pH Range

pH Minimum pH Maximum Comment Organism
5 6.5 activity is markedly reduced to about 50% at pH 5.0 and at pH 6.5, respectively Bacillus licheniformis

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 12 the enzyme shows more than 60% activity at pH 5.0-11.0 after 1 h incubation Bacillus licheniformis