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Literature summary for 3.2.1.99 extracted from

  • Shi, H.; Ding, H.; Huang, Y.; Wang, L.; Zhang, Y.; Li, X.; Wang, F.
    Expression and characterization of a GH43 endo-arabinanase from Thermotoga thermarum (2014), BMC Biotechnol., 14, 35.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.99

Activating Compound

Activating Compound Comment Organism Structure
SDS 111% activity at 0.1% SDS Pseudothermotoga thermarum
Tween 60 133% activity at 0.05% Tween 60 Pseudothermotoga thermarum

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pseudothermotoga thermarum

Inhibitors

Inhibitors Comment Organism Structure
Al3+ complete inhibition at 1 mM Pseudothermotoga thermarum
Co2+ 82% residual activity at 1 mM Pseudothermotoga thermarum
Cu2+ complete inhibition at 1 mM Pseudothermotoga thermarum
Ni2+ 31% residual activity at 1 mM Pseudothermotoga thermarum
Tris 41% residual activity at 0.05% (w/v) Pseudothermotoga thermarum
Zn2+ 14% residual activity at 1 mM Pseudothermotoga thermarum

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 113% activity at 1 mM Pseudothermotoga thermarum
Ca2+ 113% activity at 1 mM Pseudothermotoga thermarum
Mn2+ 128% activity at 1 mM Pseudothermotoga thermarum
additional information not affected by Mg2+ Pseudothermotoga thermarum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 x * 36000, SDS-PAGE Pseudothermotoga thermarum

Organism

Organism UniProt Comment Textmining
Pseudothermotoga thermarum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography Pseudothermotoga thermarum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
42.5
-
 with sugar beet arabinan as substrate, at pH 6.5 and 75°C Pseudothermotoga thermarum
199.7
-
 with debranched arabinan as substrate, at pH 6.5 and 75°C Pseudothermotoga thermarum
237.7
-
 with linear arabinan as substrate, at pH 6.5 and 75°C Pseudothermotoga thermarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
debranched arabinan + H2O
-
 Pseudothermotoga thermarum oligo-alpha-(1->5)-L-arabinoside
-
 ?
linear arabinan + H2O
-
 Pseudothermotoga thermarum oligo-alpha-(1->5)-L-arabinoside
-
 ?
additional information there is no activity towards 1,4-beta-D-mannan, galactan, 4-nitrophenyl-beta-D-xylopyranoside, and 4-nitrophenyl-alpha-L-arabinofuranoside Pseudothermotoga thermarum ?
-
 ?
sugar beet arabinan + H2O
-
 Pseudothermotoga thermarum oligo-alpha-(1->5)-L-arabinoside
-
 ?

Subunits

Subunits Comment Organism
? x * 36000, SDS-PAGE Pseudothermotoga thermarum

Synonyms

Synonyms Comment Organism
ABN
-
 Pseudothermotoga thermarum
endo-arabinanase
-
 Pseudothermotoga thermarum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
 Pseudothermotoga thermarum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 90 about 50% activity at pH 60°C, about 70% activity at pH 70°C, about 95% activity at pH 80°C, about 92% activity at pH 85°C, about 20% activity at 90°C Pseudothermotoga thermarum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70 85 the enzyme retains more than 95% of its initial activity at 70°C for 1 h. After being incubated at 80°C for 2 h, the residual activity retained is more than 80% of the initial activity. The enzyme retains more than 90% of its maximum activity at 85°C after 10 min incubation Pseudothermotoga thermarum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
349.3
-
 linear arabinan at pH 6.5 and 75°C Pseudothermotoga thermarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Pseudothermotoga thermarum

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7.5 about 35% activity at pH 5.5, about 50% activity at pH 7.5 Pseudothermotoga thermarum