Literature summary for 3.2.1.99 extracted from

de Sanctis, D.; Inacio, J.M.; Lindley, P.F.; de Sa-Nogueira, I.; Bento, I.
New evidence for the role of calcium in the glycosidase reaction of GH43 arabinanases (2010), FEBS J., 277, 4562-4574.

Search for more literature for 3.2.1.99

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) pLysS cells	Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 65% (w/v) 2-methyl-2,4-pentadiol and 100 mM Tris, pH 8.5	Bacillus subtilis

Protein Variants

Protein Variants	Comment	Organism
D171A	the mutant of isoform Arb43B displays no measurable activity	Bacillus subtilis
D38A	the mutant of isoform Arb43B displays no measurable activity	Bacillus subtilis
E224A	the mutant of isoform Arb43B displays no measurable activity	Bacillus subtilis
H318A	the mutant of isoform Arb43B displays a drastic decrease in enzymatic activity	Bacillus subtilis
H318Q	enzymatic activity is completely lost in this mutant	Bacillus subtilis

Inhibitors

Inhibitors	Comment	Organism	Structure
EGTA	isoform Arb43B shows a drastic decrease in the activity of the enzyme in the presence of 1 mm EGTA	Bacillus subtilis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.076	-	linear 1,5-alpha-L-arabinan	mutant H318A of isoform Arb43B, pH and temperature not specified in the publication	Bacillus subtilis
0.111	-	linear 1,5-alpha-L-arabinan	wild type isoform Arb43B, pH and temperature not specified in the publication	Bacillus subtilis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	isoform Arb43B possesses a cluster containing a calcium ion in the catalytic domain	Bacillus subtilis

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	P42293	-	-

Purification (Commentary)

Purification (Comment)	Organism
His-Trap column chromatography	Bacillus subtilis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
linear 1,5-alpha-L-arabinan + H2O	-	Bacillus subtilis	L-arabinose + ?	-	?

Synonyms

Synonyms	Comment	Organism
ABN	-	Bacillus subtilis
Arb43A	isoform, formally named AbnA	Bacillus subtilis
Arb43B	isoform, formally named Abn2	Bacillus subtilis
endo-1,5-alpha-L-arabinanase	-	Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.1	-	linear 1,5-alpha-L-arabinan	mutant H318A of isoform Arb43B, pH and temperature not specified in the publication	Bacillus subtilis
191.6	-	linear 1,5-alpha-L-arabinan	wild type isoform Arb43B, pH and temperature not specified in the publication	Bacillus subtilis

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.00004	-	linear 1,5-alpha-L-arabinan	mutant H318A of isoform Arb43B, pH and temperature not specified in the publication	Bacillus subtilis
0.00172	-	linear 1,5-alpha-L-arabinan	wild type isoform Arb43B, pH and temperature not specified in the publication	Bacillus subtilis

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Release 2023.1 (January 2023)