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Literature summary for 3.2.1.99 extracted from

  • Yamaguchi, A.; Tada, T.; Wada, K.; Nakaniwa, T.; Kitatani, T.; Sogabe, Y.; Takao, M.; Sakai, T.; Nishimura, K.
    Structural basis for thermostability of endo-1,5-alpha-L-arabinanase from Bacillus thermodenitrificans TS-3 (2005), J. Biochem., 137, 587-592.
    View publication on PubMed
Search for more literature for 3.2.1.99

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapor-diffusion method using 1.0 mM sodium citrate as a precipitant at pH 6.0. Structure determined at 1.9 A to an R-factor of 18.3% and an R-free-factor of 22.5%. The enzyme molecule has a five-bladed beta-propeller fold. Crystals belong to orthorhombic space group P2(1)2(1)2(1) with unit cell parameters of a = 40.3, b = 77.8 and c = 89.7 A Geobacillus thermodenitrificans

Protein Variants

Protein Variants Comment Organism
DELTAV2-W17 deletion of the N-terminal amino acids causes thermal instability Geobacillus thermodenitrificans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 x * 33000, mutant enzyme DELTAV2-W17, SDS-PAGE Geobacillus thermodenitrificans

Organism

Organism UniProt Comment Textmining
Geobacillus thermodenitrificans
-
-
-
Geobacillus thermodenitrificans TS-3
-
-
-

Subunits

Subunits Comment Organism
? x * 33000, mutant enzyme DELTAV2-W17, SDS-PAGE Geobacillus thermodenitrificans

Synonyms

Synonyms Comment Organism
endo-1,5-alpha-L-arabinanase
-
 Geobacillus thermodenitrificans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 mutant enzyme DELTAV2-W17 Geobacillus thermodenitrificans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 mutant enzyme DELTAV2-W17 loses 65% of its activity after 4 h, half-life: 2.5 h Geobacillus thermodenitrificans
65
-
 mutant enzyme DELTAV2-W17, 70% loss of activity after 5 min Geobacillus thermodenitrificans
70
-
 wild-type enzyme retains more than 80% of its activity after 4 h, complete loss of activity of mutant enzyme DELTAV2-W17 after 5 min Geobacillus thermodenitrificans
75
-
 half-life of wild-type enzyme: 4 h Geobacillus thermodenitrificans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 mutant enzyme DELTAV2-W17 Geobacillus thermodenitrificans