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Literature summary for 3.2.1.99 extracted from

  • Flipphi, M.J.A.; Panneman, H.; van der Veen, P.; Visser, J.; de Graaff, L.H.
    Molecular cloning, expression and structure of the endo-1,5-alpha-L-arabinase gene of Aspergillus niger (1993), Appl. Microbiol. Biotechnol., 40, 318-326.
    View publication on PubMed
Search for more literature for 3.2.1.99

Activating Compound

Activating Compound Comment Organism Structure
L-arabitol induction of endo-arabinase, addition of D-glucose prevents this induction Aspergillus nidulans
L-arabitol induction of endo-arabinase, addition of D-glucose prevents this induction Aspergillus niger

Cloned(Commentary)

Cloned (Comment) Organism
cDNA clone, overexpression in Aspergillus niger and Aspergillus nidulans Aspergillus niger

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Aspergillus nidulans
-
-
extracellular
-
 Aspergillus niger
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32500
-
 calculated from nucleotide sequence Aspergillus niger
43000
-
 SDS-PAGE Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus nidulans
-
 wild-type WG096
-
Aspergillus niger
-
 wild-type N400 and mutant N572, lacks xylulose kinase activity
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein O-linked glycosylation: approximately 10000 Da Aspergillus niger

Purification (Commentary)

Purification (Comment) Organism
wild-type N400 Aspergillus niger

Reaction

Reaction Comment Organism Reaction ID
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O = alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose regulation, induction and carbon catabolite repression of endo-1,5-alpha-arabinanase Aspergillus nidulans
a
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O = alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose regulation, induction and carbon catabolite repression of endo-1,5-alpha-arabinanase Aspergillus niger
a

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Aspergillus nidulans
-
culture medium
-
 Aspergillus niger
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,5-alpha-L-arabinan + H2O best substrate, endo mechanism Aspergillus niger alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
-
 ?
L-arabinan + H2O sugar beet pulp Aspergillus nidulans L-arabino-oligosaccharides
-
 ?
L-arabinan + H2O sugar beet pulp Aspergillus niger L-arabino-oligosaccharides end-product: L-arabinose disaccharide + L-arabinose trisaccharide, upon exhaustive degradation of substrate some monomers, L-arabinose, are produced ?
L-arabinan + H2O random hydrolysis of L-arabinan Aspergillus niger L-arabino-oligosaccharides end-product: L-arabinose disaccharide + L-arabinose trisaccharide, upon exhaustive degradation of substrate some monomers, L-arabinose, are produced ?

Synonyms

Synonyms Comment Organism
ABN
-
 Aspergillus niger
ABN A
-
 Aspergillus niger
endo-1,5-alpha-L-arabinase A
-
 Aspergillus niger