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Literature summary for 3.2.1.99 extracted from

  • Sakamoto, T.; Yamada, M.; Kawasaki, H.; Sakai, T.
    Molecular cloning and nucleotide sequence of an endo-1,5-alpha-L-arabinase gene from Bacillus subtilis (1997), Eur. J. Biochem., 245, 708-714.
    View publication on PubMed
Search for more literature for 3.2.1.99

Application

Application Comment Organism
analysis useful in structural analysis of primary cell wall polysaccharides Bacillus subtilis
nutrition useful for pectin extraction from citrus peel from fruit-juice industries and sugar-beet pulp from sugar factories Bacillus subtilis
synthesis applied to the refinement of cotton fiber, enzyme is able to release the cotton fiber coating, yielding product of high quality but with lower amounts of wastes Bacillus subtilis

Cloned(Commentary)

Cloned (Comment) Organism
IFO 3134 Bacillus subtilis

General Stability

General Stability Organism
IFO 3134, enzyme stable against protease secreted by the microorganism even during 2 days cultivation Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
CH3COO- NH4+ IFO 3134, complete inhibition of enzyme production Bacillus subtilis
CH3COONa IFO 3134, inhibition of enzyme production Bacillus subtilis
additional information IFO 3134, inhibition of enzyme production Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
(NH4)2HPO4 IFO 3134, stimulation of enzyme production and activity Bacillus subtilis
(NH4)H2PO4 IFO 3134, stimulation of enzyme production and activity Bacillus subtilis
K2HPO4 IFO 3134, stimulation of enzyme production and activity Bacillus subtilis
KH2PO4 IFO 3134, stimulation of enzyme production and activity Bacillus subtilis
additional information IFO 3134, stimulation of enzyme production by several phosphates, but not by chloride, acetate or sulfate Bacillus subtilis
Na2HPO4 IFO 3134, stimulation of enzyme production and activity Bacillus subtilis
NaH2PO4 IFO 3134, stimulation of enzyme production and activity Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 IFO 3134, SDS-PAGE Bacillus subtilis
35710
-
 IFO 3134, calculated from nucleotide sequence Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
 IFO 3134
-

Reaction

Reaction Comment Organism Reaction ID
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O = alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose regulation, induction and carbon catabolite repression of endo-1,5-alpha-arabinanase Bacillus subtilis
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,5-alpha-L-arabinan + H2O IFO 3134, linear 1,5-alpha-L-arabinan Bacillus subtilis alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + L-arabinose
-
 ?
L-arabinan + H2O sugar beet pulp Bacillus subtilis L-arabino-oligosaccharides
-
 ?
L-arabinan + H2O random hydrolysis of L-arabinan Bacillus subtilis L-arabino-oligosaccharides
-
 ?
protopectin + H2O IFO 3134, protopectin of sugar-beet and apple pulp Bacillus subtilis pectin IFO 3134, soluble pectin ?

Synonyms

Synonyms Comment Organism
protopectinase C IFO 3134 Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 IFO 3134 Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 IFO 3134 Bacillus subtilis