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Literature summary for 3.2.1.98 extracted from

  • Kumar, L.; Subramanian, G.; Ayyadurai, S.; Sharma, T.; Singh, S.; Meenakshi, R.
    Studies on a maltohexaose (G6) producing alkaline amylase from a novel alkalophilic Streptomyces species (2012), IIOAB J., 3, 15-30.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol
-
Streptomyces sp.
L-cysteine
-
Streptomyces sp.

Application

Application Comment Organism
food industry the enzyme preparation is effective in removing starch based stains Streptomyces sp.

Inhibitors

Inhibitors Comment Organism Structure
EDTA approximately 50% loss of activity in the presence of 2 mM EDTA Streptomyces sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ essential for activity and protein folding Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36300
-
1 * 36920 + 1 * 36300 , estimated from native PAGE Streptomyces sp.
36920
-
1 * 36920 + 1 * 36300 , estimated from native PAGE Streptomyces sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
starch + H2O Streptomyces sp.
-
maltohexaose + maltotetrose + maltotriose + maltose the predominant products are maltohexaose (48%) followed by maltotetrose, maltotriose and maltose ?

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
starch + H2O
-
Streptomyces sp. maltohexaose + maltotetrose + maltotriose + maltose the predominant products are maltohexaose (48%) followed by maltotetrose, maltotriose and maltose ?

Subunits

Subunits Comment Organism
heterodimer 1 * 36920 + 1 * 36300 , estimated from native PAGE Streptomyces sp.

Synonyms

Synonyms Comment Organism
maltohexaose producing alkaline amylase
-
Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Streptomyces sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 50
-
Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Streptomyces sp.

pH Range

pH Minimum pH Maximum Comment Organism
7 10
-
Streptomyces sp.

pI Value

Organism Comment pI Value Maximum pI Value
Streptomyces sp. isoelectric focusing
-
6.3

Expression

Organism Comment Expression
Streptomyces sp. soluble starch as well as raw starch induce the enzyme expression, induction being higher with raw starch up