Application | Comment | Organism |
---|---|---|
industry | high compatibility and performance of engineered alpha-amylase AmyUS100DIG/M197A, suggesting its potential application in detergent industry | Geobacillus stearothermophilus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutant AmyUS100DELTAIG with improved thermostability | Geobacillus stearothermophilus |
additional information | mutant AmyUS100DELTAIG/M197A shows alterations in the specific activity and the profile of starch hydrolysis. Incubation in the presence of Triton X-100 causes the decrease of 30% of the initial activity. Surfactant SDS and LAS cause a moderate denaturation of the activity by 55% and 30%, respectively. Incubation of the enzyme at 40°C with Ariel, Skip, Dixan, Det, NewDet, Nadhif and OMO increases the activity between 10% and 50%. Significantly lower requirement for calcium ions, high resistance to thermal inactivation, to chelators, to protease and to oxidative additives, in conjunction with conserved activity after storage | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Geobacillus stearothermophilus US100 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by affinity purification | Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
845 | - |
mutant AmyUS100DELTAIG/M197A | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | - |
Geobacillus stearothermophilus | maltohexaose + ? | - |
? | |
starch + H2O | - |
Geobacillus stearothermophilus US100 | maltohexaose + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AmyUS100 | - |
Geobacillus stearothermophilus |
maltohexaose forming alpha-amylase | - |
Geobacillus stearothermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | - |
Geobacillus stearothermophilus |