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Literature summary for 3.2.1.98 extracted from

  • Nakakuki, T.; Kainuma, K
    exo-Maltohexaohydrolase. Recent development of studies on the exo-maltohexaohydrolase (1982), J. Jpn. Soc. Starch Sci., 29, 138-144.
No PubMed abstract available
Search for more literature for 3.2.1.98

General Stability

General Stability Organism
Ca2+ stabilizes, enzyme is more thermostable in the presence of Ca2+ Klebsiella pneumoniae

Localization

Localization Comment Organism GeneOntology No. Textmining
cell bound
-
 Klebsiella pneumoniae
-
-
extracellular ultraviolet induced mutant Klebsiella pneumoniae
-
-
extracellular extracellular enzyme has the same characteristics as the cell-bound enzyme: enzyme is secreted extracellulary due to mutation of parent strain of Klebsiella pneumoniae Klebsiella pneumoniae
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 cell-bound and extracellular enzyme, gel filtration Klebsiella pneumoniae
65000
-
 extracellular enzyme, SDS-PAGE Klebsiella pneumoniae
67000
-
 cell-bound enzyme, SDS-PAGE Klebsiella pneumoniae

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
 Aerobacter aerogenes
-
Klebsiella pneumoniae
-
 ultraviolet-induced mutant
-
Klebsiella pneumoniae
-
 IFO-3321
-
Klebsiella pneumoniae IFO-3321
-
 IFO-3321
-

Purification (Commentary)

Purification (Comment) Organism
-
 Klebsiella pneumoniae

Reaction

Reaction Comment Organism Reaction ID
(Glcalpha(1-4))m + H2O = (Glcalpha(1-4))m-6 + maltohexaose reaction mechanism is exo Klebsiella pneumoniae
a

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
 Klebsiella pneumoniae
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
18.4
-
 extracellular enzyme Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin beta-amylase limit dextrin + H2O
-
 Klebsiella pneumoniae oligosaccharide
-
 ?
amylopectin beta-amylase limit dextrin + H2O
-
 Klebsiella pneumoniae IFO-3321 oligosaccharide
-
 ?
glycogen beta-amylase limit dextrin + H2O
-
 Klebsiella pneumoniae oligosaccharide
-
 ?
glycogen beta-amylase limit dextrin + H2O
-
 Klebsiella pneumoniae IFO-3321 oligosaccharide
-
 ?

Synonyms

Synonyms Comment Organism
G6-forming amylase
-
 Klebsiella pneumoniae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
52
-
 extracellular enzyme Klebsiella pneumoniae
60
-
 cell-bound enzyme Klebsiella pneumoniae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 enzyme more thermostable in the presence of Ca2+ Klebsiella pneumoniae
50
-
 cell-bound and extracellular enzyme, stable below 50°C, thermostability improved by addition of Ca2+ Klebsiella pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
 cell-bound enzyme Klebsiella pneumoniae
7
-
 extracellular enzyme Klebsiella pneumoniae

pH Range

pH Minimum pH Maximum Comment Organism
5 10 extracellular enzyme, more than 20% activity at pH 5.0, more than 50% activity at pH 10.0 Klebsiella pneumoniae

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
 pH stability: extracellular enzyme is a little more stable compared with the cell-bound enzyme Klebsiella pneumoniae
additional information
-
 extracellular enzyme, relatively wide pH stability Klebsiella pneumoniae
5 10 extracellular enzyme, more than 80% of activity retains Klebsiella pneumoniae
6 9 cell-bound enzyme: 20% loss of activity, 60 min, 40°C Klebsiella pneumoniae