Application | Comment | Organism |
---|---|---|
synthesis | continuous production of maltohexaose on large scale using the immobilized exomaltohexaohydrolase | Klebsiella pneumoniae |
General Stability | Organism |
---|---|
immobilized enzyme is very stable | Klebsiella pneumoniae |
immobilized enzyme stable to repeated use of 20 cycles | Klebsiella pneumoniae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AgNO3 | native and immobilized enzyme, immobilized enzyme less than native enzyme | Klebsiella pneumoniae | |
Al2(SO4)3 | immobilized enzyme, slight inactivation | Klebsiella pneumoniae | |
Ba2+ | immobilized enzyme | Klebsiella pneumoniae | |
Cu2+ | immobilized enzyme less than native enzyme | Klebsiella pneumoniae | |
FeCl3 | immobilized enzyme | Klebsiella pneumoniae | |
FeSO4 | native and immobilized enzyme | Klebsiella pneumoniae | |
Hg2+ | immobilized enzyme, 61% inactivation; native enzyme, completely | Klebsiella pneumoniae | |
HgCl2 | immobilized enzyme, 61% inactivation; native enzyme, completely | Klebsiella pneumoniae | |
iodoacetamide | native enzyme: 11% inhibition, immobilized enzyme: 4% inhibition | Klebsiella pneumoniae | |
Mg2+ | immobilized enzyme, slight inactivation | Klebsiella pneumoniae | |
Mn2+ | native enzyme, slight inactivation | Klebsiella pneumoniae | |
additional information | native enzyme compared with immobilized enzyme | Klebsiella pneumoniae | |
p-chloromercuribenzoate | native enzyme: 46% inhibition, immobilized enzyme: 15% inhibition | Klebsiella pneumoniae | |
SrCl2 | immobilized enzyme | Klebsiella pneumoniae | |
Zn2+ | native enzyme | Klebsiella pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | native enzyme compared with immobilized enzyme, Km-value of native and immobilized enzyme for short-chain amylose is the same, for soluble starch Km-value of immobilized enzyme is larger than that of native enzyme | Klebsiella pneumoniae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | ultraviolet induced mutant | Klebsiella pneumoniae | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | native enzyme, activation | Klebsiella pneumoniae | |
Ca2+ | immobilized enzyme, no activation | Klebsiella pneumoniae | |
CoCl2 | native and immobilized enzyme, very slight activation | Klebsiella pneumoniae | |
Mn2+ | immobilized enzyme, activation | Klebsiella pneumoniae | |
additional information | native enzyme compared with immobilized enzyme | Klebsiella pneumoniae | |
NiCl2 | immobilized enzyme, slight activation | Klebsiella pneumoniae | |
Sr2+ | native enzyme, very slight activation | Klebsiella pneumoniae | |
Zn2+ | immobilized enzyme, very slight activation | Klebsiella pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | - |
Aerobacter aerogenes | - |
Klebsiella pneumoniae | - |
native and immobilized, by irradiation method, enzyme | - |
Klebsiella pneumoniae | - |
ultraviolet-induced mutant | - |
Purification (Comment) | Organism |
---|---|
partial | Klebsiella pneumoniae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15.6 | - |
- |
Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylose + H2O | short-chain amylose, DP, average degree of polymerization, = 23 | Klebsiella pneumoniae | maltohexaose | main product, about 38% maltohexaose | ? | |
maltohexaose + H2O | - |
Klebsiella pneumoniae | maltose + maltotetraose | - |
? | |
maltohexaose + H2O | - |
Klebsiella pneumoniae | D-glucose + maltopentaose | - |
? | |
maltohexaose + H2O | - |
Klebsiella pneumoniae | maltotriose + ? | - |
? | |
additional information | action pattern on substrates of native and immobilized enzyme is almost the same | Klebsiella pneumoniae | ? | - |
? | |
starch + H2O | - |
Klebsiella pneumoniae | maltohexaose + ? | main product | ? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
native enzyme compared with immobilized enzyme | Klebsiella pneumoniae |
52 | - |
immobilized enzyme | Klebsiella pneumoniae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
native enzyme compared with immobilized enzyme | Klebsiella pneumoniae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
thermostability is improved by immobilization | Klebsiella pneumoniae |
50 | - |
immobilized enzyme, completely stable up to | Klebsiella pneumoniae |
75 | - |
immobilized enzyme, 15 min at 75°C: 40% of activity retained | Klebsiella pneumoniae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
native enzyme compared with immobilized enzyme, immobilized enzyme: optimum pH shifted slightly to acidic side | Klebsiella pneumoniae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
native enzyme compared with immobilized enzyme | Klebsiella pneumoniae |
5 | 8 | immobilized enzyme, more than 80% of maximum activity between 5.0 and 8.0 | Klebsiella pneumoniae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH stability is improved by immobilization | Klebsiella pneumoniae |
3 | - |
immobilized enzyme, 93% of activity retains | Klebsiella pneumoniae |
4 | 11 | immobilized enzyme, stable between | Klebsiella pneumoniae |
11.5 | - |
immobilized enzyme, 85% of activity retained | Klebsiella pneumoniae |