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Literature summary for 3.2.1.96 extracted from
- Transglycosylation activity of glycosynthase mutants of endo-beta-N-acetylglucosaminidase from Coprinopsis cinerea (2015), PLoS ONE, 10, e0132859 .
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | the enzyme is a useful tool for analyzing oligosaccharide contents of glycoproteins. Endo-CC1 would potentially be a valuable tool for analyzing oligosaccharides on glycoproteins, as large quantities of it can be made available easily and less economically | Coprinopsis cinerea |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Coprinopsis cinerea |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| N180A | 0.5% of the activity compared to wild-type enzyme | Coprinopsis cinerea |
| N180C | 2.1% of the activity compared to wild-type enzyme | Coprinopsis cinerea |
| N180D | 5.8% of the activity compared to wild-type enzyme | Coprinopsis cinerea |
| N180E | 3.3% of the activity compared to wild-type enzyme | Coprinopsis cinerea |
| N180F | 0.1% of the activity compared to wild-type enzyme | Coprinopsis cinerea |
| N180G | 0.4% of the activity compared to wild-type enzyme | Coprinopsis cinerea |
| N180H | 8.7% of the activity compared to wild-type enzyme. Wild-type Endo-CC1 can not transfer the complex type sialobiantennary oligosaccharide onto the GlcNAc-RNase B. The mutant enzyme transfers the oligosaccharide onto the GlcNAc-RNase B. This protein is named Neo-RNase B. The N180H mutant does not cleave the oligosaccharide off the Neo-RNase B | Coprinopsis cinerea |
| N180I | 4.0% of the activity compared to wild-type enzyme | Coprinopsis cinerea |
| N180K | 0.2% of the activity compared to wild-type enzyme | Coprinopsis cinerea |
| N180L | 0.6% of the activity compared to wild-type enzyme | Coprinopsis cinerea |
| N180M | 12.0% of the activity compared to wild-type enzyme | Coprinopsis cinerea |
| N180P | 0.8% of the activity compared to wild-type enzyme | Coprinopsis cinerea |
| N180Q | 16.9% of the activity compared to wild-type enzyme. Wild-type Endo-CC1 can not transfer the complex type sialobiantennary oligosaccharide onto the GlcNAc-RNase B. The mutant enzyme transfers the oligosaccharide onto the GlcNAc-RNase B. This protein is named Neo-RNase B. The N180Q mutant cleaves the oligosaccharide off the Neo-RNase B | Coprinopsis cinerea |
| N180R | no activity | Coprinopsis cinerea |
| N180S | 1.0% of the activity compared to wild-type enzyme | Coprinopsis cinerea |
| N180T | 1.0% of the activity compared to wild-type enzyme | Coprinopsis cinerea |
| N180V | 3.4% of the activity compared to wild-type enzyme | Coprinopsis cinerea |
| N180W | no activity | Coprinopsis cinerea |
| N180Y | no activity | Coprinopsis cinerea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Coprinopsis cinerea | A8P7P2 | - |
- |
| Coprinopsis cinerea | D6RKV4 | - |
- |
| Coprinopsis cinerea Okayama-7 | A8P7P2 | - |
- |
| Coprinopsis cinerea Okayama-7 | D6RKV4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| expression of the His6-tagged enzyme in Escherichia coli BL21-CodonPlus (DE3) | Coprinopsis cinerea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (dansyl)-labeled Man5GlcNAc2-Asn + H2O | hydrolytic activity of endo-beta-N-acetylglucosaminidase Endo-CC1 is higher than that of Endo-CC2 | Coprinopsis cinerea | ? | - |
? |  |
| (dansyl)-labeled Man5GlcNAc2-Asn + H2O | hydrolytic activity of endo-beta-N-acetylglucosaminidase Endo-CC1 on (dansyl)-labeled Man5GlcNAc2-Asn is higher than that of Endo-CC2 | Coprinopsis cinerea | ? | - |
? | |
| (dansyl)-labeled Man5GlcNAc2-Asn + H2O | hydrolytic activity of endo-beta-N-acetylglucosaminidase Endo-CC1 is higher than that of Endo-CC2 | Coprinopsis cinerea Okayama-7 | ? | - |
? | |
| (dansyl)-labeled Man5GlcNAc2-Asn + H2O | hydrolytic activity of endo-beta-N-acetylglucosaminidase Endo-CC1 on (dansyl)-labeled Man5GlcNAc2-Asn is higher than that of Endo-CC2 | Coprinopsis cinerea Okayama-7 | ? | - |
? | |
| alpha-NeuAc-(2->6)-beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)[alpha-NeuAc-(2->6)-beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)]beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-Asn + H2O | hydrolytic activity of endo-beta-N-acetylglucosaminidase Endo-CC1 is higher than that of Endo-CC2 | Coprinopsis cinerea | ? | - |
? | |
| alpha-NeuAc-(2->6)-beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)[alpha-NeuAc-(2->6)-beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)]beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-Asn + H2O | hydrolytic activity of endo-beta-N-acetylglucosaminidase Endo-CC1 is higher than that of Endo-CC2 | Coprinopsis cinerea Okayama-7 | ? | - |
? | |
| additional information | endo-beta-N-acetylglucosaminidase Endo-CC1 does not act on the sialotriantennary type complex oligosaccharide of glycoprotein fetuin. Endo-CC1 can not transfer the sialobiantennary type complex oligosaccharide onto the deglycosylated RNase B | Coprinopsis cinerea | ? | - |
? | |
| additional information | endo-beta-N-acetylglucosaminidase Endo-CC1 does not act on the sialotriantennary type complex oligosaccharide of glycoprotein fetuin. Endo-CC1 can not transfer the sialobiantennary type complex oligosaccharide onto the deglycosylated RNase B | Coprinopsis cinerea Okayama-7 | ? | - |
? | |
| N-glycosylated human transferrin + H2O | endo-beta-N-acetylglucosaminidase Endo-CC1 acts on both N-linked high-mannose type and sialobiantennary type complex oligosaccharides of the glycoprotein human transferrin | Coprinopsis cinerea | ? | - |
? | |
| N-glycosylated human transferrin + H2O | endo-beta-N-acetylglucosaminidase Endo-CC1 acts on both N-linked high-mannose type and sialobiantennary type complex oligosaccharides of the glycoprotein human transferrin | Coprinopsis cinerea Okayama-7 | ? | - |
? | |
| N-glycosylated RNase B + H2O | endo-beta-N-acetylglucosaminidase Endo-CC1 acts on both N-linked high-mannose type and sialobiantennary type complex oligosaccharides of the glycoprotein RNase B | Coprinopsis cinerea | ? | - |
? | |
| N-glycosylated RNase B + H2O | endo-beta-N-acetylglucosaminidase Endo-CC1 acts on both N-linked high-mannose type and sialobiantennary type complex oligosaccharides of the glycoprotein RNase B | Coprinopsis cinerea Okayama-7 | ? | - |
? | |
| Neu2Gal2GlcNAc2-Man3GlcNAc2-Asn + H2O | hydrolytic activity of endo-beta-N-acetylglucosaminidase Endo-CC1 on Neu2Gal2GlcNAc2-Man3GlcNAc2-Asn is higher than that of Endo-CC2 | Coprinopsis cinerea | ? | - |
? | |
| Neu2Gal2GlcNAc2-Man3GlcNAc2-Asn + H2O | hydrolytic activity of endo-beta-N-acetylglucosaminidase Endo-CC1 on Neu2Gal2GlcNAc2-Man3GlcNAc2-Asn is higher than that of Endo-CC2 | Coprinopsis cinerea Okayama-7 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Endo-CC1 | - |
Coprinopsis cinerea |
| Endo-CC2 | - |
Coprinopsis cinerea |
| ENGase | - |
Coprinopsis cinerea |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
10 min, stable | Coprinopsis cinerea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Coprinopsis cinerea |
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