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Literature summary for 3.2.1.96 extracted from

  • Garrido, D.; Nwosu, C.; Ruiz-Moyano, S.; Aldredge, D.; German, J.B.; Lebrilla, C.B.; Mills, D.A.
    Endo-beta-N-acetylglucosaminidases from infant gut-associated bifidobacteria release complex N-glycans from human milk glycoproteins (2012), Mol. Cell. Proteomics, 11, 775-785.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.96

Protein Variants

Protein Variants Comment Organism
D184N the mutated enzyme specifically binds to the core of N-glycans, Man3GlcNAc2 Bifidobacterium longum subsp. infantis
D184N b1 the mutated enzyme specifically binds to the core of N-glycans, Man3GlcNAc2 Bifidobacterium longum subsp. Longum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 x * 47000, isoform EndoBI-1, calculated from amino acid sequence Bifidobacterium longum subsp. infantis
53000
-
 x * 53000, isoform EndoBI-2, calculated from amino acid sequence Bifidobacterium longum subsp. infantis
98000
-
 x * 98000, calculated from amino acid sequence Bifidobacterium longum subsp. Longum

Organism

Organism UniProt Comment Textmining
Bifidobacterium longum subsp. infantis
-
-
-
Bifidobacterium longum subsp. infantis ATCC 15697
-
-
-
Bifidobacterium longum subsp. infantis SC142
-
-
-
Bifidobacterium longum subsp. Longum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is active toward all major types of N-linked glycans found in glycosylated proteins. Its activity is not affected by core fucosylation or extensive fucosylation, antenna number, or sialylation. Extensive N-deglycosylation of whole breast milk is also observed after coincubation with this enzyme Bifidobacterium longum subsp. infantis ?
-
 ?
additional information the enzyme is active toward all major types of N-linked glycans found in glycosylated proteins. Its activity is not affected by core fucosylation or extensive fucosylation, antenna number, or sialylation. Extensive N-deglycosylation of whole breast milk is also observed after coincubation with this enzyme Bifidobacterium longum subsp. infantis SC142 ?
-
 ?
additional information the enzyme is active toward all major types of N-linked glycans found in glycosylated proteins. Its activity is not affected by core fucosylation or extensive fucosylation, antenna number, or sialylation. Extensive N-deglycosylation of whole breast milk is also observed after coincubation with this enzyme Bifidobacterium longum subsp. infantis ATCC 15697 ?
-
 ?
N-glycosylated bovine lactoferrin + H2O
-
 Bifidobacterium longum subsp. infantis ?
-
 ?
N-glycosylated bovine lactoferrin + H2O
-
 Bifidobacterium longum subsp. Longum ?
-
 ?
N-glycosylated bovine lactoferrin + H2O highest activity Bifidobacterium longum subsp. infantis ?
-
 ?
N-glycosylated bovine lactoferrin + H2O
-
 Bifidobacterium longum subsp. infantis SC142 ?
-
 ?
N-glycosylated bovine lactoferrin + H2O highest activity Bifidobacterium longum subsp. infantis SC142 ?
-
 ?
N-glycosylated bovine lactoferrin + H2O
-
 Bifidobacterium longum subsp. infantis ATCC 15697 ?
-
 ?
N-glycosylated bovine lactoferrin + H2O highest activity Bifidobacterium longum subsp. infantis ATCC 15697 ?
-
 ?
N-glycosylated bovine serum albumin + H2O
-
 Bifidobacterium longum subsp. infantis ?
-
 ?
N-glycosylated bovine serum albumin + H2O
-
 Bifidobacterium longum subsp. Longum ?
-
 ?
N-glycosylated bovine serum albumin + H2O lowest activity Bifidobacterium longum subsp. infantis ?
-
 ?
N-glycosylated bovine serum albumin + H2O
-
 Bifidobacterium longum subsp. infantis SC142 ?
-
 ?
N-glycosylated bovine serum albumin + H2O
-
 Bifidobacterium longum subsp. infantis ATCC 15697 ?
-
 ?
N-glycosylated human lactoferrin + H2O
-
 Bifidobacterium longum subsp. infantis ?
-
 ?
N-glycosylated human lactoferrin + H2O
-
 Bifidobacterium longum subsp. Longum ?
-
 ?
N-glycosylated immunoglobulin A + H2O
-
 Bifidobacterium longum subsp. infantis ?
-
 ?
N-glycosylated immunoglobulin A + H2O
-
 Bifidobacterium longum subsp. Longum ?
-
 ?
N-glycosylated immunoglobulin G + H2O
-
 Bifidobacterium longum subsp. infantis ?
-
 ?
N-glycosylated immunoglobulin G + H2O
-
 Bifidobacterium longum subsp. Longum ?
-
 ?
N-glycosylated immunoglobulin G + H2O
-
 Bifidobacterium longum subsp. infantis SC142 ?
-
 ?
N-glycosylated immunoglobulin G + H2O
-
 Bifidobacterium longum subsp. infantis ATCC 15697 ?
-
 ?
N-glycosylated RNase B + H2O
-
 Bifidobacterium longum subsp. infantis ?
-
 ?
N-glycosylated RNase B + H2O
-
 Bifidobacterium longum subsp. Longum ?
-
 ?
N-glycosylated RNase B + H2O second highest activity Bifidobacterium longum subsp. infantis ?
-
 ?

Subunits

Subunits Comment Organism
? x * 47000, isoform EndoBI-1, calculated from amino acid sequence Bifidobacterium longum subsp. infantis
? x * 53000, isoform EndoBI-2, calculated from amino acid sequence Bifidobacterium longum subsp. infantis
? x * 98000, calculated from amino acid sequence Bifidobacterium longum subsp. Longum

Synonyms

Synonyms Comment Organism
endo-beta-N-acetylglucosaminidase
-
 Bifidobacterium longum subsp. infantis
endo-beta-N-acetylglucosaminidase
-
 Bifidobacterium longum subsp. Longum
EndoBB
-
 Bifidobacterium longum subsp. Longum
EndoBI-1 isoform Bifidobacterium longum subsp. infantis
EndoBI-2 isoform Bifidobacterium longum subsp. infantis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 45
-
 Bifidobacterium longum subsp. infantis
30 45
-
 Bifidobacterium longum subsp. Longum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
95
-
 the enzymatic activity of isoform EndoBI-1 is not significantly impaired by incubation at 95 °C for 1 or 5 min Bifidobacterium longum subsp. infantis
95
-
 the enzymatic activity of isoform EndoBI-2 is not significantly impaired by incubation at 95°C for 1 or 5 min Bifidobacterium longum subsp. infantis
95
-
 the enzymatic activity of the enzyme is significantly impaired by incubation at 95 °C for 1 or 5 min Bifidobacterium longum subsp. Longum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Bifidobacterium longum subsp. infantis
5
-
-
 Bifidobacterium longum subsp. Longum