Literature summary for 3.2.1.96 extracted from

Frisch, E.; Schwedler, C.; Kaup, M.; Iona Braicu, E.; Groene, J.; Lauscher, J.C.; Sehouli, J.; Zimmermann, M.; Tauber, R.; Berger, M.; Blanchard, V.
Endo-beta-N-acetylglucosaminidase H de-N-glycosylation in a domestic microwave oven: application to biomarker discovery (2013), Anal. Biochem., 433, 65-69.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Streptomyces plicatus

Organism

Organism	UniProt	Comment	Textmining
Streptomyces plicatus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the enzyme cleaves high-mannose and hybrid-type N-glycans between the first two GlcNAc residues at the reducing end	Streptomyces plicatus	?	-	?
N-glycosylated human serum + H2O	-	Streptomyces plicatus	Man5GlcNAc + ?	main product	?
N-glycosylated RNase B + H2O	-	Streptomyces plicatus	Man5GlcNAc + ?	main product	?

Synonyms

Synonyms	Comment	Organism
Endo H	-	Streptomyces plicatus
endo-beta-N-acetylglucosaminidase H	-	Streptomyces plicatus

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Release 2023.1 (January 2023)