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Literature summary for 3.2.1.96 extracted from

  • Umekawa, M.; Huang, W.; Li, B.; Fujita, K.; Ashida, H.; Wang, L.X.; Yamamoto, K.
    Mutants of Mucor hiemalis endo-{beta}-N-acetylglucosaminidase show enhanced transglycosylation and glycosynthase-like activities (2008), J. Biol. Chem., 283, 4469-4479.
    View publication on PubMed
Search for more literature for 3.2.1.96

Application

Application Comment Organism
medicine use of enzyme for synthesis of a human immunodeficiency virus type 1 glycopeptide with potent anti-HIV activity Mucor hiemalis
synthesis use of enzyme for synthesis of a human immunodeficiency virus type 1 glycopeptide with potent anti-HIV activity Mucor hiemalis

Protein Variants

Protein Variants Comment Organism
E177A complete loss of activities Mucor hiemalis
N175A mutant acts like a glycosynthase. Mutation knocks out the hydrolytic activity, but mutant takes sugar oxazolines as donor substrates for transglycosylation Mucor hiemalis
N230S reduction in transglycosylation activity Mucor hiemalis
N249S almost complete loss of transglycosylation activity Mucor hiemalis
W228F reduction in transglycosylation activity Mucor hiemalis
W251N almost complete loss of transglycosylation activity Mucor hiemalis
Y217F enhanced transglycosylation activity and diminished hydrolytic activity Mucor hiemalis
Y217F/W228F reduction in transglycosylation activity Mucor hiemalis
Y250N almost complete loss of transglycosylation activity Mucor hiemalis

Organism

Organism UniProt Comment Textmining
Mucor hiemalis
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00039
-
 mutant N175A, hydrolytic activity, pH 6.25, 30°C Mucor hiemalis
0.0017
-
 mutant N249S, transglycosylation activity, pH 6.25, 30°C Mucor hiemalis
0.0018
-
 mutant W251N, transglycosylation activity, pH 6.25, 30°C Mucor hiemalis
0.0027
-
 mutant Y250N, transglycosylation activity, pH 6.25, 30°C Mucor hiemalis
0.023
-
 mutant N230S, transglycosylation activity, pH 6.25, 30°C Mucor hiemalis
0.023
-
 mutant Y217F/W228F, transglycosylation activity, pH 6.25, 30°C Mucor hiemalis
0.026
-
 mutant W228F, transglycosylation activity, pH 6.25, 30°C Mucor hiemalis
0.058
-
 wild-type, transglycosylation activity, pH 6.25, 30°C Mucor hiemalis
0.085
-
 mutant Y217F, transglycosylation activity, pH 6.25, 30°C Mucor hiemalis
0.3
-
 mutant Y250N, hydrolytic activity, pH 6.25, 30°C Mucor hiemalis
0.32
-
 mutant N249S, hydrolytic activity, pH 6.25, 30°C Mucor hiemalis
0.43
-
 mutant W251N, hydrolytic activity, pH 6.25, 30°C Mucor hiemalis
0.46
-
 mutant W228F, hydrolytic activity, pH 6.25, 30°C Mucor hiemalis
0.62
-
 mutant Y217F/W228F, hydrolytic activity, pH 6.25, 30°C Mucor hiemalis
0.7
-
 mutant N230S, hydrolytic activity, pH 6.25, 30°C Mucor hiemalis
1.2
-
 mutant Y217F, hydrolytic activity, pH 6.25, 30°C Mucor hiemalis
1.6
-
 wild-type, hydrolytic activity, pH 6.25, 30°C Mucor hiemalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-beta-D-N-acetylglucosaminide + H2O
-
 Mucor hiemalis 4-methylumbelliferone + beta-D-N-acetylglucosamine
-
 ?
dansyl-Lys-Val-Ala-Asn((NeuAc-Gal-GlcNAc-Man)2Man-GlcNAc2)-Lys-Thr + H2O
-
 Mucor hiemalis ?
-
 ?
Lys-Val-Ala-Asn((NeuAc-Gal-GlcNAc-Man)2Man-GlcNAc2)-Lys-Thr + 4-methylumbelliferyl-beta-D-N-acetylglucosaminide
-
 Mucor hiemalis ?
-
 ?