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Literature summary for 3.2.1.96 extracted from

  • Fujita, K.; Sato, R.; Toma, K.; Kitahara, K.; Suganuma, T.; Yamamoto, K.; Takegawa, K.
    Identification of the catalytic acid base residue of Arthrobacter endo-beta-N-acetylglucosaminidase by chemical rescue of an inactive mutant (2007), J. Biochem., 142, 301-306.
    View publication on PubMed
Search for more literature for 3.2.1.96

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Glutamicibacter protophormiae

Protein Variants

Protein Variants Comment Organism
E173A mutation in catalytically essential residue. Partial rescue of activity by addition of sodium azide or sodium formate, the produced beta-glycosyl azide retaines the anomeric configuration Glutamicibacter protophormiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.207
-
 dansyl-Asn-(GlcNAc)2(Man)5 wild-type, pH 6.0, 37°C Glutamicibacter protophormiae
0.876
-
 dansyl-Asn-(GlcNAc)2(Man)5 mutant E174A, presence of 2 M sodium azide, pH 6.0, 37°C Glutamicibacter protophormiae

Organism

Organism UniProt Comment Textmining
Glutamicibacter protophormiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dansyl-Asn-(GlcNAc)2(Man)5 + H2O
-
 Glutamicibacter protophormiae dansyl-Asn-GlcNAc + ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0458
-
 dansyl-Asn-(GlcNAc)2(Man)5 mutant E174A, presence of 2 M sodium azide, pH 6.0, 37°C Glutamicibacter protophormiae
365
-
 dansyl-Asn-(GlcNAc)2(Man)5 wild-type, pH 6.0, 37°C Glutamicibacter protophormiae