Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli TOP10, enzymes with truncation or point mutation | Streptococcus pneumoniae |
Protein Variants | Comment | Organism |
---|---|---|
del316/delY319/E320 | mutation completely removes the activity for (Man)5(GlcNAc)2Asn, a low level of activity towards (Man)3(GlcNAc)2Asn remains | Streptococcus pneumoniae |
delE324 | loss of activity | Streptococcus pneumoniae |
delW359/delY360 | loss of activity | Streptococcus pneumoniae |
additional information | truncated enzyme form in which 134 N-terminal and 599-C-tterminal amino acids are deleted, still retains enzymatic activity. The specificity of the truncated enzyme is indistinguishable from the intact enzyme and also acts on the core structure of the asparagine-linked oligosaccharides attached to intact IgG | Streptococcus pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pneumoniae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
complex-type aspargine-linked oligosaccharide + H2O | hydrolysis of the di-N-acetylchitobiose structure. The specificity for complex type oligosaccharides is probably defined by multiple domains in Endo D structure | Streptococcus pneumoniae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Endo D | - |
Streptococcus pneumoniae |
endo-beta-N-acetylglucosaminidase D | - |
Streptococcus pneumoniae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
5 min, about 90% loss of activity | Streptococcus pneumoniae |
55 | - |
5 min, about 65% loss of activity | Streptococcus pneumoniae |