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Literature summary for 3.2.1.96 extracted from

  • Chalifour, R.J.; Spiro, R.G.
    Cleavage of dolichyl pyrophosphoryl oligosaccharides by endo-beta-N-acetylglucosaminidase H: comparison of enzymatic and acid hydrolysis techniques for saccharide release (1984), Arch. Biochem. Biophys., 229, 386-394.
    View publication on PubMed
Search for more literature for 3.2.1.96

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 maximal stimulation at 0.03%, or small amounts of other detergents Streptomyces griseus

Application

Application Comment Organism
analysis useful in metabolic studies dealing with oligosaccharide-lipid assembly and their involvement in the N-glycosylation of proteins Streptomyces griseus

Inhibitors

Inhibitors Comment Organism Structure
SDS 0.1%, strong inhibition Streptomyces griseus

Organism

Organism UniProt Comment Textmining
Streptomyces griseus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dolichyl pyrophosphoryl oligosaccharides + H2O both glucosylated and unglucosylated polymannose oligosaccharides are released by the enzyme through cleavage of the di-N-acetylchitobiose squence Streptomyces griseus ?
-
 ?
additional information specificity Streptomyces griseus ?
-
 ?
additional information the rate of hydrolysis of lipid-linked (Glc)3(Man)9(GlcNAc)2 is substantially slower than that of the same oligosaccharide attached to asparagine in a peptide sequence Streptomyces griseus ?
-
 ?

Synonyms

Synonyms Comment Organism
endo-beta-N-acetylglucosaminidase H
-
 Streptomyces griseus