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Literature summary for 3.2.1.94 extracted from
- Crystal structure and mutational analysis of isomalto-dextranase, a member of glycoside hydrolase family 27 (2015), J. Biol. Chem., 290, 26339-26349 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21(DE3) | Arthrobacter globiformis |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| crystallized at 20°C using the hanging drop vapor diffusion method | Arthrobacter globiformis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D207A | 4fold decrease in kcat/Km for 4-nitrophenyl alpha-isomaltoside as compared to wild-type value. 28fold decrease in kcat/Km for dextran T2000 as compared to wild-type value | Arthrobacter globiformis |
| D207A/N209A | 4.2fold decrease in kcat/Km for 4-nitrophenyl alpha-isomaltoside as compared to wild-type value. 17.8fold decrease in kcat/Km for dextran T2000 as compared to wild-type value | Arthrobacter globiformis |
| M243A | 233fold decrease in kcat/Km for 4-nitrophenyl alpha-isomaltoside as compared to wild-type value. 334fold decrease in kcat/Km for dextran T2000 as compared to wild-type value | Arthrobacter globiformis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.093 | - |
dextran T2000 | pH 5.3, 30°C, mutant D207A | Arthrobacter globiformis | |
| 0.103 | - |
dextran T2000 | pH 5.3, 30°C, wild-type enzyme | Arthrobacter globiformis | |
| 0.106 | - |
dextran T2000 | pH 5.3, 30°C, mutant M243A | Arthrobacter globiformis | |
| 0.109 | - |
dextran T2000 | pH 5.3, 30°C, mutant D207A/N209A | Arthrobacter globiformis | |
| 65.1 | - |
4-nitrophenyl alpha-isomaltoside | pH 5.3, 30°C, wild-type enzyme | Arthrobacter globiformis |  |
| 95.1 | - |
4-nitrophenyl alpha-isomaltoside | pH 5.3, 30°C, mutant D207A | Arthrobacter globiformis | |
| 108 | - |
4-nitrophenyl alpha-isomaltoside | pH 5.3, 30°C, mutant D207A/N209A | Arthrobacter globiformis | |
| 125 | - |
4-nitrophenyl alpha-isomaltoside | pH 5.3, 30°C, mutant M243A | Arthrobacter globiformis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arthrobacter globiformis | Q7WSN5 | - |
- |
| Arthrobacter globiformis T6 | Q7WSN5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Arthrobacter globiformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-isomaltoside + H2O | - |
Arthrobacter globiformis | 4-nitrophenol + alpha-isomaltose | - |
? | |
| 4-nitrophenyl alpha-isomaltoside + H2O | - |
Arthrobacter globiformis T6 | 4-nitrophenol + alpha-isomaltose | - |
? | |
| dextran T2000 + H2O | the enzyme liberates isomaltose from the non-reducing end of a polymer of dextran. It does not show alpha-N-galactosidase or alpha-N-acetylgalactosaminidase activities | Arthrobacter globiformis | isomaltose + ? | - |
? | |
| dextran T2000 + H2O | the enzyme liberates isomaltose from the non-reducing end of a polymer of dextran. It does not show alpha-N-galactosidase or alpha-N-acetylgalactosaminidase activities | Arthrobacter globiformis T6 | isomaltose + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AgIMD | - |
Arthrobacter globiformis |
| isomalto-dextranase | - |
Arthrobacter globiformis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Arthrobacter globiformis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.194 | - |
4-nitrophenyl alpha-isomaltoside | pH 5.3, 30°C, mutant M243A | Arthrobacter globiformis | |
| 1.03 | - |
dextran T2000 | pH 5.3, 30°C, mutant M243A | Arthrobacter globiformis | |
| 1.66 | - |
dextran T2000 | pH 5.3, 30°C, mutant D207A | Arthrobacter globiformis | |
| 3.06 | - |
dextran T2000 | pH 5.3, 30°C, mutant D207A/N209A | Arthrobacter globiformis | |
| 8.7 | - |
4-nitrophenyl alpha-isomaltoside | pH 5.3, 30°C, mutant D207A | Arthrobacter globiformis | |
| 9.26 | - |
4-nitrophenyl alpha-isomaltoside | pH 5.3, 30°C, mutant D207A/N209A | Arthrobacter globiformis | |
| 23.6 | - |
4-nitrophenyl alpha-isomaltoside | pH 5.3, 30°C, wild-type enzyme | Arthrobacter globiformis | |
| 51.6 | - |
dextran T2000 | pH 5.3, 30°C, wild-type enzyme | Arthrobacter globiformis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.3 | - |
assay at | Arthrobacter globiformis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.56 | - |
4-nitrophenyl alpha-isomaltoside | pH 5.3, 30°C, mutant M243A | Arthrobacter globiformis | |
| 10.5 | - |
dextran T2000 | pH 5.3, 30°C, mutant M243A | Arthrobacter globiformis | |
| 17.9 | - |
dextran T2000 | pH 5.3, 30°C, mutant D207A | Arthrobacter globiformis | |
| 28.1 | - |
dextran T2000 | pH 5.3, 30°C, mutant D207A/N209A | Arthrobacter globiformis | |
| 86.1 | - |
4-nitrophenyl alpha-isomaltoside | pH 5.3, 30°C, mutant D207A/N209A | Arthrobacter globiformis | |
| 91.5 | - |
4-nitrophenyl alpha-isomaltoside | pH 5.3, 30°C, mutant D207A | Arthrobacter globiformis | |
| 363 | - |
4-nitrophenyl alpha-isomaltoside | pH 5.3, 30°C, wild-type enzyme | Arthrobacter globiformis | |
| 501 | - |
dextran T2000 | pH 5.3, 30°C, wild-type enzyme | Arthrobacter globiformis |
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