Literature summary for 3.2.1.91 extracted from

Haq, I.U.; Tahir, S.F.; Aftab, M.N.; Akram, F.; Asad-Ur-Rehman, F.; Nawaz, A.; Mukhtar, H.
Purification and characterization of a thermostable cellobiohydrolase from Thermotoga petrophila (2018), Protein Pept. Lett., 25, 1003-1014 .

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Cloned(Commentary)

Cloned (Comment)	Organism
-	Thermotoga petrophila

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	-	Thermotoga petrophila
Fe2+	-	Thermotoga petrophila
Hg2+	-	Thermotoga petrophila
Pb2+	-	Thermotoga petrophila

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
4.6	-	4-nitrophenyl beta-D-cellobioside	pH and temperature not specified in the publication	Thermotoga petrophila

Organism

Organism	UniProt	Comment	Textmining
Thermotoga petrophila	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation and ion exchange chromatography	Thermotoga petrophila

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
66.5	-	after 4.05fold purification, pH and temperature not specified in the publication	Thermotoga petrophila

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl beta-D-cellobioside + H2O	-	Thermotoga petrophila	4-nitrophenol + cellobiose	-	?

Subunits

Subunits	Comment	Organism
?	x * 37000, SDS-PAGE	Thermotoga petrophila

Synonyms

Synonyms	Comment	Organism
cellobiohydrolase	-	Thermotoga petrophila

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
90	-	the enzyme shows 50% residual activity at 90°C	Thermotoga petrophila

pH Range

pH Minimum	pH Maximum	Comment	Organism
4	10	the enzyme shows 50% residual activity pH range of 4.0-10.0	Thermotoga petrophila

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Release 2023.1 (January 2023)