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Literature summary for 3.2.1.91 extracted from

  • Lee, C.R.; Chi, W.J.; Lim, J.H.; Dhakshnamoorthy, V.; Hong, S.K.
    Expression and characterization of the processive exo-beta-1,4-cellobiohydrolase SCO6546 from Streptomyces coelicolor A(3) (2018), J. Basic Microbiol., 58, 310-321 .
    View publication on PubMed
Search for more literature for 3.2.1.91

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Streptomyces lividans strain TK24 Streptomyces coelicolor
expressed in Streptomyces lividans TK24 cells Streptomyces coelicolor

Inhibitors

Inhibitors Comment Organism Structure
Co2+ about 40% residual activity at 5 mM; the addition of 5 mM CoCl2 inhibits enzyme activity by 59% Streptomyces coelicolor
Cu2+ about 80% residual activity at 5 mM; the addition of 5 mM CuCl2 inhibits enzyme activity by 21% Streptomyces coelicolor
Mn2+ about 30% residual activity at 5 mM; the addition of 5 mM MnCl2 inhibits enzyme activity by 64% Streptomyces coelicolor
additional information EDTA (5 mM) does not affect the enzyme activity; the addition of EDTA (5 mM) does not affect enzyme activity Streptomyces coelicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7
-
 cellotetraose at pH 5.0 and 50°C Streptomyces coelicolor

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ the addition of CaCl2 enhances enzyme activity by 159% Streptomyces coelicolor
Ca2+ about 159% activity at 5 mM Streptomyces coelicolor
additional information the enzyme does not require a metal cofactor Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cellulose + H2O Streptomyces coelicolor the enzyme acts on nonreducing end of cellulose cellobiose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces coelicolor
-
-
-
Streptomyces coelicolor O86728
-
-
Streptomyces coelicolor A(3)
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and Superdex 200HR gel filtration Streptomyces coelicolor
Superdex 200 gel filtration Streptomyces coelicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl cellopentaoside + H2O
-
 Streptomyces coelicolor cellobiose + 4-nitrophenyl cellotrioside
-
 ?
4-nitrophenyl cellotetraoside + H2O
-
 Streptomyces coelicolor cellobiose + 4-nitrophenyl cellobioside
-
 ?
agarose + H2O low activity Streptomyces coelicolor ?
-
 ?
agarose + H2O about 40% activity compared to Avicel Streptomyces coelicolor ?
-
 ?
avicel + H2O highest activity Streptomyces coelicolor cellobiose
-
 ?
avicel + H2O high activity Streptomyces coelicolor cellobiose
-
 ?
beechwood xylan + H2O low activity Streptomyces coelicolor ?
-
 ?
beechwood xylan + H2O about 20% activity compared to Avicel Streptomyces coelicolor ?
-
 ?
beta-glucan + H2O low activity Streptomyces coelicolor ?
-
 ?
beta-glucan + H2O low activity Streptomyces coelicolor cellobiose
-
 ?
carboxymethyl cellulose + H2O high activity Streptomyces coelicolor cellobiose + ?
-
 ?
carboxymethyl cellulose + H2O 97% activity compared to Avicel Streptomyces coelicolor cellobiose
-
 ?
cellohexaose + H2O
-
 Streptomyces coelicolor cellobiose + cellotetraose + cellotriose
-
 ?
cellopentaose + H2O
-
 Streptomyces coelicolor cellobiose + cellotriose
-
 ?
cellotetraose + H2O
-
 Streptomyces coelicolor 2 cellobiose
-
 ?
cellotetraose + H2O
-
 Streptomyces coelicolor A(3) 2 cellobiose
-
 ?
cellulose + H2O the enzyme acts on nonreducing end of cellulose Streptomyces coelicolor cellobiose + ?
-
 ?
filter paper + H2O low activity Streptomyces coelicolor ?
-
 ?
filter paper + H2O about 18% activity compared to Avicel Streptomyces coelicolor ?
-
 ?
galactan + H2O low activity Streptomyces coelicolor ?
-
 ?
galactan + H2O about 30% activity compared to Avicel Streptomyces coelicolor ?
-
 ?
galactan + H2O about 30% activity compared to Avicel Streptomyces coelicolor A(3) ?
-
 ?
melibiose + H2O about 4% activity compared to Avicel Streptomyces coelicolor ?
-
 ?
additional information the enzyme completely lacks chromogenic activity on the 4-nitrophenyl-cellodextrins from 4-nitrophenyl glucoside to 4-nitrophenyl cellobioside Streptomyces coelicolor ?
-
 ?
additional information no activity with cellotriose, alpha-cellulose, cellobiose, and raffinose. The enzyme completely lacks chromogenic activity on the 4-nitrophenyl cellodextrins from 4-nitrophenyl glucoside to 4-nitrophenyl cellobioside Streptomyces coelicolor ?
-
 ?
additional information no activity with cellotriose, alpha-cellulose, cellobiose, and raffinose. The enzyme completely lacks chromogenic activity on the 4-nitrophenyl cellodextrins from 4-nitrophenyl glucoside to 4-nitrophenyl cellobioside Streptomyces coelicolor A(3) ?
-
 ?
oat spelt xylan + H2O about 2% activity compared to Avicel Streptomyces coelicolor ?
-
 ?
stachyose + H2O about 18% activity compared to Avicel Streptomyces coelicolor ?
-
 ?
stachyose + H2O about 18% activity compared to Avicel Streptomyces coelicolor A(3) ?
-
 ?
xyloglucan + H2O low activity Streptomyces coelicolor ?
-
 ?
xyloglucan + H2O about 37% activity compared to Avicel Streptomyces coelicolor ?
-
 ?
xyloglucan + H2O about 37% activity compared to Avicel Streptomyces coelicolor A(3) ?
-
 ?

Subunits

Subunits Comment Organism
? x * 103400, calculated from amino acid sequence Streptomyces coelicolor
? x * 99400, mature secreted protein, SDS-PAGE Streptomyces coelicolor
? x * 99400, recombinant enzyme, SDS-PAGE Streptomyces coelicolor

Synonyms

Synonyms Comment Organism
cellobiohydrolase
-
 Streptomyces coelicolor
exo-beta-1,4-cellobiohydrolase
-
 Streptomyces coelicolor
SCO6546
-
 Streptomyces coelicolor

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Streptomyces coelicolor

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60 the enzyme shows more than 55% of its maximum activity at 30-60 °C Streptomyces coelicolor
30 60 the enzyme retains more than 55% of its maximum activity at 30-60°C Streptomyces coelicolor

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 60 the enzyme is stable up to 50°C, but is dramatically inactivated at 60°C (8% activity remaining after 1 h) Streptomyces coelicolor
50 60 the enzyme is stable up to 50°C, but is dramatically inactivated at 60°C (8% activity remaining after 1 h) Streptomyces coelicolor

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
13.3
-
 cellotetraose at pH 5.0 and 50°C Streptomyces coelicolor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Streptomyces coelicolor
5
-
 pH optimum using Avicel or cellotetraose as substrates Streptomyces coelicolor
6
-
 pH optimum using cellopentaose as substrate Streptomyces coelicolor

pH Range

pH Minimum pH Maximum Comment Organism
5 7 more than 50% activity between pH 5.0 and 7.0 Streptomyces coelicolor
5 7 more than 50% enzyme activity toward Avicel at pH 5.0-7.0 Streptomyces coelicolor

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 9 the enzyme is stable over a wide pH range from 5.0-7.0, however, more than 75% of the cellulase activity is lost at pH 4.0 and 9.0 after 1 h Streptomyces coelicolor