Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.91 extracted from

  • Chen, Y.C.; Chen, W.T.; Liu, J.C.; Tsai, L.C.; Cheng, H.L.
    A highly active beta-glucanase from a new strain of rumen fungus Orpinomyces sp. Y102 exhibits cellobiohydrolase and cellotriohydrolase activities (2014), Biores. Technol., 170, 513-521.
    View publication on PubMed
Search for more literature for 3.2.1.91

Cloned(Commentary)

Cloned (Comment) Organism
-
 Orpinomyces sp.

Protein Variants

Protein Variants Comment Organism
additional information CelC7 is insoluble when expressed in Escherichia coli. Deletion of 17 or 105 residues from the N-terminus significantly improves its solubility Orpinomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 Barley beta-glucan Km value 2.18 mg/ml, 105-residue N-terminal truncation mutant, pH 6.0, 45°C Orpinomyces sp.
additional information
-
 Barley beta-glucan Km value 3.03 mg/ml, 17-residue N-terminal truncation mutant, pH 6.0, 45°C Orpinomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51500
-
 x * 51500, SDS-PAGE Orpinomyces sp.

Organism

Organism UniProt Comment Textmining
Orpinomyces sp.
-
-
-
Orpinomyces sp. Y102
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification sequence contains a signal peptide (residues 1-17) Orpinomyces sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20.3
-
 substrate acid-swollen avicel, 17-residue N-terminal truncation mutant, pH 6.0, 45°C Orpinomyces sp.
26
-
 substrate carboxymethyl cellulose, 17-residue N-terminal truncation mutant, pH 6.0, 45°C Orpinomyces sp.
4564
-
 substrate barley beta-glucan, 17-residue N-terminal truncation mutant, pH 6.0, 45°C Orpinomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acid-swollen avicel + H2O
-
 Orpinomyces sp. cellobiose + cellotriose
-
 ?
acid-swollen avicel + H2O
-
 Orpinomyces sp. Y102 cellobiose + cellotriose
-
 ?
barley beta-glucan + H2O
-
 Orpinomyces sp. ?
-
 ?
barley beta-glucan + H2O
-
 Orpinomyces sp. Y102 ?
-
 ?
carboxymethyl cellulose + H2O
-
 Orpinomyces sp. ?
-
 ?
carboxymethyl cellulose + H2O
-
 Orpinomyces sp. Y102 ?
-
 ?
cellohexaose + H2O
-
 Orpinomyces sp. cellobiose + cellotriose + cellotetraose enzyme does not release D-glucose ?
cellohexaose + H2O
-
 Orpinomyces sp. Y102 cellobiose + cellotriose + cellotetraose enzyme does not release D-glucose ?
additional information no substrates: avicel, soluble oat spelt xylan, Pachyman, 4-nitrophenyl-beta-D-cellobioside, 4-nitrophenyl-beta-D-galactopyranoside Orpinomyces sp. ?
-
 ?
additional information no substrates: avicel, soluble oat spelt xylan, Pachyman, 4-nitrophenyl-beta-D-cellobioside, 4-nitrophenyl-beta-D-galactopyranoside Orpinomyces sp. Y102 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 51500, SDS-PAGE Orpinomyces sp.

Synonyms

Synonyms Comment Organism
CelC7
-
 Orpinomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 17-residue N-terminal truncation mutant Orpinomyces sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50 more than 70% of maximum activity, 17-residue N-terminal truncation mutant Orpinomyces sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4223
-
 Barley beta-glucan 105-residue N-terminal truncation mutant, pH 6.0, 45°C Orpinomyces sp.
4402
-
 Barley beta-glucan 17-residue N-terminal truncation mutant, pH 6.0, 45°C Orpinomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Orpinomyces sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 11
-
 Orpinomyces sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
 Barley beta-glucan Km value is 2.18 mg/ml, pH 6.0, 45°C, 17-residue N-terminal truncation mutant Orpinomyces sp.