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Literature summary for 3.2.1.91 extracted from

  • Lee, K.M.; Joo, A.R.; Jeya, M.; Lee, K.M.; Moon, H.J.; Lee, J.K.
    Production and characterization of cellobiohydrolase from a novel strain of Penicillium purpurogenum KJS506 (2011), Appl. Biochem. Biotechnol., 163, 25-39.
    View publication on PubMed
Search for more literature for 3.2.1.91

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 136.5% activity at 0.5 mM Talaromyces purpureogenus

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 33.7% inhibition at 1 mM Talaromyces purpureogenus
EDTA 24.6% inhibition at 1 mM Talaromyces purpureogenus
Fe2+ complete inhibition at 1 mM Talaromyces purpureogenus
Hg2+ complete inhibition at 1 mM Talaromyces purpureogenus
Zn2+ 21.2% inhibition at 1 mM Talaromyces purpureogenus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1
-
 4-nitrophenyl beta-D-cellobioside at pH 5.0, 60°C Talaromyces purpureogenus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 1 * 60000, SDS-PAGE Talaromyces purpureogenus
61000
-
 gel filtration Talaromyces purpureogenus

Organism

Organism UniProt Comment Textmining
Talaromyces purpureogenus
-
-
-
Talaromyces purpureogenus KJS506
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose column chromatography, Mono Q column chromatography, and Sephacryl S-300 gel filtration Talaromyces purpureogenus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6
-
 crude enzyme, pH 5.0, 60°C Talaromyces purpureogenus
10.8
-
 after4.2fold purification, pH 5.0, 60°C Talaromyces purpureogenus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-cellobioside + H2O best substrate Talaromyces purpureogenus 4-nitrophenol + cellobiose
-
 ?
4-nitrophenyl beta-D-cellobioside + H2O best substrate Talaromyces purpureogenus KJS506 4-nitrophenol + cellobiose
-
 ?
avicel + H2O
-
 Talaromyces purpureogenus ?
-
 ?
avicel + H2O
-
 Talaromyces purpureogenus KJS506 ?
-
 ?
cellopentaose + H2O
-
 Talaromyces purpureogenus cellobiose + ? cellobiose is the major product ?
cellopentaose + H2O
-
 Talaromyces purpureogenus KJS506 cellobiose + ? cellobiose is the major product ?
cellotetraose + H2O
-
 Talaromyces purpureogenus cellobiose + ? cellobiose is the major product ?
filter paper + H2O
-
 Talaromyces purpureogenus ?
-
 ?
filter paper + H2O
-
 Talaromyces purpureogenus KJS506 ?
-
 ?
additional information does not hydrolyze carboxymethylcellulose Talaromyces purpureogenus ?
-
 ?
additional information does not hydrolyze carboxymethylcellulose Talaromyces purpureogenus KJS506 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 60000, SDS-PAGE Talaromyces purpureogenus

Synonyms

Synonyms Comment Organism
CBH
-
 Talaromyces purpureogenus
cellobiohydrolase
-
 Talaromyces purpureogenus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 activity decreases rapidly at temperatures over 60°C Talaromyces purpureogenus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 the enzyme shows a half-life of 72 h, 46 h, 22 h, 4 h, and 0.5 h at 30°C, 40°C, 50°C, 60°C, and 70 °C, respectively Talaromyces purpureogenus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Talaromyces purpureogenus

pH Range

pH Minimum pH Maximum Comment Organism
4 7 82% and 79% of the maximum activity at pH 4.0 and and pH 6.0 Talaromyces purpureogenus

pI Value

Organism Comment pI Value Maximum pI Value
Talaromyces purpureogenus isoelectric focusing
-
 4.8