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Literature summary for 3.2.1.91 extracted from

  • Heinzelman, P.; Snow, C.D.; Smith, M.A.; Yu, X.; Kannan, A.; Boulware, K.; Villalobos, A.; Govindarajan, S.; Minshull, J.; Arnold, F.H.
    SCHEMA recombination of a fungal cellulase uncovers a single mutation that contributes markedly to stability (2009), J. Biol. Chem., 284, 26229-26233.
    View publication on PubMedView publication on EuropePMC
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Protein Variants

Protein Variants Comment Organism
C313S random mutagenesis, the mutation causes increased thermostability of the mutant enzyme compared to the wild-type, with decreased inactivation, increased maximum Avicel hydrolysis temperature, and improved long time hydrolysis performance Phanerodontia chrysosporium
C313S random mutagenesis, the mutation causes increased thermostability of the mutant enzyme compared to the wild-type, with decreased inactivation, increased maximum Avicel hydrolysis temperature, and improved long time hydrolysis performance Humicola insolens
C313S random mutagenesis, the mutation causes increased thermostability of the mutant enzyme compared to the wild-type, with decreased inactivation, increased maximum Avicel hydrolysis temperature, and improved long time hydrolysis performance. The C313S mutation increases total Humicola jecorina CBH II activity secreted by the Saccharomyces cerevisiae expression host more than 10fold Trichoderma reesei

Organism

Organism UniProt Comment Textmining
Humicola insolens Q9C1S9
-
-
Phanerodontia chrysosporium
-
-
-
Trichoderma reesei P07987
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
avicel + H2O microcrystalline cellulose Phanerodontia chrysosporium ?
-
 ?
avicel + H2O microcrystalline cellulose Trichoderma reesei ?
-
 ?
avicel + H2O microcrystalline cellulose Humicola insolens ?
-
 ?

Synonyms

Synonyms Comment Organism
CBH II
-
 Phanerodontia chrysosporium
CBH II
-
 Trichoderma reesei
CBH II
-
 Humicola insolens
cellobiohydrolase class II
-
 Phanerodontia chrysosporium
cellobiohydrolase class II
-
 Trichoderma reesei
cellobiohydrolase class II
-
 Humicola insolens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 assay at Phanerodontia chrysosporium
50
-
 assay at Trichoderma reesei
50
-
 assay at Humicola insolens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.8
-
 assay at Phanerodontia chrysosporium
4.8
-
 assay at Trichoderma reesei
4.8
-
 assay at Humicola insolens