Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Trichoderma reesei |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method | Trichoderma reesei |
Protein Variants | Comment | Organism |
---|---|---|
D241C/D249C | thermostability similar to wild-type enzyme | Trichoderma reesei |
DELTAG245-Y252 | reduced thermostability | Trichoderma reesei |
Y247F | slight reduction of kcat on 4-nitrophenyl lactoside, but only a small effect on cellulose hydrolysis | Trichoderma reesei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cellobiose | competitive inhibitor with 4-nitrophenyl lactoside as substrate | Trichoderma reesei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
4-nitrophenyl lactoside | pH 5, 30°C, Y247F mutant | Trichoderma reesei | |
0.041 | - |
4-nitrophenyl lactoside | pH 5, 30°C, wild-type enzyme | Trichoderma reesei | |
0.44 | - |
4-nitrophenyl lactoside | pH 5, 30°C, D241C/D249C mutant | Trichoderma reesei | |
1.8 | - |
4-nitrophenyl lactoside | pH 5, 30°C, DELTAG245-Y252 mutant | Trichoderma reesei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichoderma reesei | P62694 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-lactoside + H2O | - |
Trichoderma reesei | 4-nitrophenol + ? | - |
? | |
cellulose + H2O | - |
Trichoderma reesei | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.061 | - |
4-nitrophenyl lactoside | pH 5, 30°C, Y247F mutant | Trichoderma reesei | |
0.093 | - |
4-nitrophenyl lactoside | pH 5, 30°C, wild-type enzyme | Trichoderma reesei | |
0.19 | - |
4-nitrophenyl lactoside | pH 5, 30°C, DELTAG245-Y252 mutant | Trichoderma reesei | |
0.5 | - |
cellotetraose | pH 5, 27°C, wild-type enzyme | Trichoderma reesei | |
0.58 | - |
cellotetraose | pH 5, 27°C, Y247F mutant and D241C/D249C mutant | Trichoderma reesei | |
0.82 | - |
4-nitrophenyl lactoside | pH 5, 30°C, D241C/D249C mutant | Trichoderma reesei | |
0.83 | - |
cellotetraose | pH 5, 27°C, DELTAG245-Y252 mutant | Trichoderma reesei |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
22 | - |
cellobiose | pH 5, 30°C, Y247F mutant | Trichoderma reesei | |
24 | - |
cellobiose | pH 5, 30°C, wild-type enzyme | Trichoderma reesei | |
300 | - |
cellobiose | pH 5, 30°C, DELTAG245-Y252 mutant | Trichoderma reesei |