Literature summary for 3.2.1.86 extracted from

Kwan, D.H.; Jin, Y.; Jiang, J.; Chen, H.M.; Koetzler, M.P.; Overkleeft, H.S.; Davies, G.J.; Withers, S.G.
Chemoenzymatic synthesis of 6-phospho-cyclophellitol as a novel probe of 6-phospho-beta-glucosidases (2016), FEBS Lett., 590, 461-468 .

Search for more literature for 3.2.1.86

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Streptococcus pyogenes

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with 6-phospho-cyclophellitol, sitting drop vapor diffusion method, using 20% (w/v) PEG3350, NaBr 0.15 M, Bis-Tris propane 0.1 M, pH 7.5	Streptococcus pyogenes

Inhibitors

Inhibitors	Comment	Organism	Structure
6-phospho-cyclophellitol	-	Streptococcus pyogenes

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.73	-	4-nitrophenyl 6-phospho-beta-glucoside	at pH 6.0 and 37°C	Streptococcus pyogenes

Organism

Organism	UniProt	Comment	Textmining
Streptococcus pyogenes	Q99YP9	-	-
Streptococcus pyogenes M1	Q99YP9	-	-

Purification (Commentary)

Purification (Comment)	Organism
HisTrap column chromatography	Streptococcus pyogenes

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl 6-phospho-beta-glucoside + H2O	-	Streptococcus pyogenes	4-nitrophenol + D-glucose 6-phosphate	-	?
4-nitrophenyl 6-phospho-beta-glucoside + H2O	-	Streptococcus pyogenes M1	4-nitrophenol + D-glucose 6-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
SPy1599	-	Streptococcus pyogenes

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.24	-	4-nitrophenyl 6-phospho-beta-glucoside	at pH 6.0 and 37°C	Streptococcus pyogenes

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
2.4	-	6-phospho-cyclophellitol	at pH 6.0 and 37°C	Streptococcus pyogenes

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Release 2023.1 (January 2023)