BRENDA - Enzyme Database show
show all sequences of 3.2.1.86

Structural basis for enzyme bifunctionality - the case of Gan1D from Geobacillus stearothermophilus

Lansky, S.; Zehavi, A.; Belrhali, H.; Shoham, Y.; Shoham, G.; FEBS J. 284, 3931-3953 (2017)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
in complex with cellobiose-6-phosphate, 6-phospho-D-galactose or 6-phospho-D-glucose, hanging drop vapor diffusion method, using 16-22% (w/v) polyethylene glycol 8 K, 3% (w/v) methylpentanediol, 0.1 M imidazole buffer pH 6.5
Geobacillus stearothermophilus
Engineering
Amino acid exchange
Commentary
Organism
E170Q
catalytic mutant
Geobacillus stearothermophilus
W433A
the mutation shifts the enzyme specificity from dual activity to a significant preference toward 6-phospho-beta-glucosidase activity
Geobacillus stearothermophilus
W433M
the mutation shifts the enzyme specificity from dual activity to a significant preference toward 6-phospho-beta-glucosidase activity
Geobacillus stearothermophilus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
115000
-
gel filtration
Geobacillus stearothermophilus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Geobacillus stearothermophilus
W8QF82
-
-
Geobacillus stearothermophilus T-1
W8QF82
-
-
Purification (Commentary)
Commentary
Organism
-
Geobacillus stearothermophilus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
lactose 6-phosphate + H2O
-
750522
Geobacillus stearothermophilus
beta-D-galactose-6-phosphate + D-glucose
-
-
-
?
lactose 6-phosphate + H2O
-
750522
Geobacillus stearothermophilus T-1
beta-D-galactose-6-phosphate + D-glucose
-
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
2 * 55204, calculated from amino acid sequence
Geobacillus stearothermophilus
Crystallization (Commentary) (protein specific)
Crystallization
Organism
in complex with cellobiose-6-phosphate, 6-phospho-D-galactose or 6-phospho-D-glucose, hanging drop vapor diffusion method, using 16-22% (w/v) polyethylene glycol 8 K, 3% (w/v) methylpentanediol, 0.1 M imidazole buffer pH 6.5
Geobacillus stearothermophilus
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
E170Q
catalytic mutant
Geobacillus stearothermophilus
W433A
the mutation shifts the enzyme specificity from dual activity to a significant preference toward 6-phospho-beta-glucosidase activity
Geobacillus stearothermophilus
W433M
the mutation shifts the enzyme specificity from dual activity to a significant preference toward 6-phospho-beta-glucosidase activity
Geobacillus stearothermophilus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
115000
-
gel filtration
Geobacillus stearothermophilus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Geobacillus stearothermophilus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
lactose 6-phosphate + H2O
-
750522
Geobacillus stearothermophilus
beta-D-galactose-6-phosphate + D-glucose
-
-
-
?
lactose 6-phosphate + H2O
-
750522
Geobacillus stearothermophilus T-1
beta-D-galactose-6-phosphate + D-glucose
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 55204, calculated from amino acid sequence
Geobacillus stearothermophilus
Expression
Organism
Commentary
Expression
Geobacillus stearothermophilus
the enzyme expression is upregulated by D-galactose
up
Expression (protein specific)
Organism
Commentary
Expression
Geobacillus stearothermophilus
the enzyme expression is upregulated by D-galactose
up
Other publictions for EC 3.2.1.86
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
750522
Lansky
Structural basis for enzyme b ...
Geobacillus stearothermophilus, Geobacillus stearothermophilus T-1
FEBS J.
284
3931-3953
2017
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751192
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Kwan
Chemoenzymatic synthesis of 6 ...
Streptococcus pyogenes, Streptococcus pyogenes M1
FEBS Lett.
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2
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1
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750733
Terra
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Streptococcus pneumoniae
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84
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2016
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1
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2
1
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-
-
750963
Zangoui
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2015
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4
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Stepper
Structure and activity of the ...
Streptococcus pyogenes, Streptococcus pyogenes M1 GAS SF370
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2013
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1
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1
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1
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3
3
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1
1
731178
Yang
Characterization of a novel me ...
uncultured organism
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2013
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1
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732101
Yu
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13
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6
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3
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1
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6
1
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2
2
-
6
6
732252
Honda
Purification and characterizat ...
Lactobacillus gasseri, Lactobacillus gasseri ATCC 33323
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58
11-17
2012
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8
1
2
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2
2
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1
2
-
2
2
715955
Capaldo
-
beta-Glucoside metabolism in O ...
Oenococcus oeni
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27-34
2011
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705622
Hall
Evolution and biochemistry of ...
Erwinia rhapontici, Erwinia rhapontici DSM 448
Mol. Biol. Evol.
26
2487-2497
2009
1
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1
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3
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1
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4
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678941
Vilei
beta-D-Glucoside utilization b ...
Mycoplasma mycoides
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31
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2
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681716
Honda
beta-Galactosidase, phospho-be ...
Lactobacillus acidophilus, Lactobacillus acidophilus JCM 1132T, Lactobacillus amylovorus, Lactobacillus casei, Lactobacillus crispatus, Lactobacillus fermentum, Lactobacillus gasseri, Lactobacillus johnsonii, Lactobacillus mucosae
Lett. Appl. Microbiol.
45
461-466
2007
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14
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1
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678190
Yip
Mechanistic analysis of the un ...
Thermotoga maritima
Biochemistry
45
571-580
2006
-
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11
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11
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10
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11
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666041
Varrot
NAD+ and Metal-ion Dependent H ...
Thermotoga maritima
J. Mol. Biol.
346
423-435
2005
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1
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666900
An
Structural and biochemical ana ...
Pectobacterium carotovorum
Res. Microbiol.
156
145-153
2005
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1
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1
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1
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655795
Yip
An unusual mechanism of glycos ...
Thermotoga maritima
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126
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3
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656717
Weber
Expression of the phospho-beta ...
Lactobacillus delbrueckii
Microbiology
146
1941-1948
2000
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136928
Thompson
Cellobiose-6-phosphate hydrola ...
Escherichia coli
J. Bacteriol.
181
7339-7345
1999
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7
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2
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2
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6
1
1
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7
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6
1
1
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136927
Thompson
Phospho-beta-glucosidase from ...
Fusobacterium mortiferum
J. Bacteriol.
179
1636-1645
1997
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1
9
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3
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3
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1
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11
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1
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9
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11
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1
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1
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136929
Simons
Integration and gene replaceme ...
Lactococcus lactis
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175
5168-5175
1993
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1
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136922
Schnetz
beta-Glucoside (bgl) operon of ...
Escherichia coli
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169
2579-2590
1987
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5
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2
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136923
Anderson
Phosphocellobiase ...
Klebsiella aerogenes
Methods Enzymol.
42 C
494-497
1975
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7
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1
1
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1
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1
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1
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8
-
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
1
1
-
-
-
1
-
-
1
-
8
-
-
-
-
-
1
-
-
-
-
-
-
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-
-
136924
Wilson
The beta-glucoside system of E ...
Escherichia coli
J. Biol. Chem.
249
5586-5598
1974
-
-
-
-
-
-
10
9
-
-
4
-
-
1
-
-
1
-
-
-
1
2
6
1
-
-
2
-
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
10
-
9
-
-
4
-
-
-
-
1
-
-
1
2
6
1
-
-
2
-
2
1
1
-
-
-
-
-
-
-
136926
Prasad
Genetic determination of the c ...
Escherichia coli
J. Bacteriol.
114
909-915
1973
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-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
136925
Palmer
Cellobiose metabolism in Aerob ...
Klebsiella aerogenes
J. Biol. Chem.
247
3420-3423
1972
-
-
-
-
-
-
-
7
-
-
1
1
-
1
-
-
1
-
-
-
1
1
8
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
1
1
-
-
-
1
-
-
1
1
8
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-