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Literature summary for 3.2.1.86 extracted from

  • Honda, H.; Nagaoka, S.; Kawai, Y.; Kemperman, R.; Kok, J.; Yamazaki, Y.; Tateno, Y.; Kitazawa, H.; Saito, T.
    Purification and characterization of two phospho-?-galactosidases, LacG1 and LacG2, from Lactobacillus gasseri ATCC33323 (2012), J. Gen. Appl. Microbiol., 58, 11-17.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
LacG1, phylogenetic analysis Lactobacillus gasseri
LacG2, phylogenetic analysis, recombinant expression of His6-tagged enzyme in Lactococcus lactis strain MG1363 Lactobacillus gasseri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.66
-
2-nitrophenyl-beta-D-glucopyranoside 6-phosphate pH 7.0, 40°C Lactobacillus gasseri
0.97
-
2-nitrophenyl-beta-D-glucopyranoside 6-phosphate pH 6.0, 50°C Lactobacillus gasseri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
x * 55000, recombinant enzyme, SDS-PAGE Lactobacillus gasseri

Organism

Organism UniProt Comment Textmining
Lactobacillus gasseri O06532
-
-
Lactobacillus gasseri ATCC 33323 O06532
-
-

Purification (Commentary)

Purification (Comment) Organism
native LacG1 to homogeneity by ammonium sulfate fractionation, dialysis, anion exchange and hydroxyapatite chromatography, and ultrafiltration Lactobacillus gasseri
recombinant His6-tagged enzyme LacG2 to homogeneity by nickel affinity and adsorption chromatography, and ultrafiltration Lactobacillus gasseri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
-
Lactobacillus gasseri 2-nitrophenol + beta-D-glucose 6-phosphate
-
?
2-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
-
Lactobacillus gasseri ATCC 33323 2-nitrophenol + beta-D-glucose 6-phosphate
-
?
additional information the enzyme also shows 6-phospho-beta-galactosidase activity, EC 3.2.1.85 Lactobacillus gasseri ?
-
?
additional information the enzyme also shows 6-phospho-beta-galactosidase activity, EC 3.2.1.85 Lactobacillus gasseri ATCC 33323 ?
-
?

Subunits

Subunits Comment Organism
? x * 55000, recombinant enzyme, SDS-PAGE Lactobacillus gasseri

Synonyms

Synonyms Comment Organism
LacG1
-
Lactobacillus gasseri
LacG2
-
Lactobacillus gasseri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Lactobacillus gasseri
50
-
-
Lactobacillus gasseri

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2 8 2-nitrophenyl-beta-D-glucopyranoside 6-phosphate pH 7.0, 40°C Lactobacillus gasseri
41
-
2-nitrophenyl-beta-D-glucopyranoside 6-phosphate pH 6.0, 50°C Lactobacillus gasseri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Lactobacillus gasseri
7
-
-
Lactobacillus gasseri

General Information

General Information Comment Organism
metabolism the enzyme is involved in the metabolic pathway of lactose in Lactobacillus gasseri, overview Lactobacillus gasseri

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
44
-
2-nitrophenyl-beta-D-glucopyranoside 6-phosphate pH 7.0, 40°C Lactobacillus gasseri
59
-
2-nitrophenyl-beta-D-glucopyranoside 6-phosphate pH 7.0, 40°C Lactobacillus gasseri