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Literature summary for 3.2.1.86 extracted from

  • Yip, V.L.; Withers, S.G.
    Mechanistic analysis of the unusual redox-elimination sequence employed by Thermotoga maritima BglT: a 6-phospho-beta-glucosidase from glycoside hydrolase family 4 (2006), Biochemistry, 45, 571-580.
    View publication on PubMed
Search for more literature for 3.2.1.86

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 4-chloro-2-nitrophenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima
0.0166
-
 2,5-dinitrophenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima
0.0174
-
 2-nitrophenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima
0.0314
-
 3,4-dinitrophenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima
0.0319
-
 4-cyanophenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima
0.0414
-
 3,5-dichlorophenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima
0.0444
-
 2,4-dinitrophenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima
0.0454
-
 4-cyanophenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima
0.048
-
 4-nitrophenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima
0.0583
-
 4-tert-butylphenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima
0.0729
-
 3-nitrophenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-dinitrophenyl 6-phospho-beta-D-glucoside + H2O
-
 Thermotoga maritima 2,4-dinitrophenol + D-glucose 6-phosphate
-
 ?
2,5-dinitrophenyl 6-phospho-beta-D-glucoside + H2O
-
 Thermotoga maritima 2,5-dinitrophenol + D-glucose 6-phosphate
-
 ?
2-nitrophenyl 6-phospho-beta-D-glucoside + H2O
-
 Thermotoga maritima 2-nitrophenol + D-glucose 6-phosphate
-
 ?
3,4-dinitrophenyl 6-phospho-beta-D-glucoside + H2O
-
 Thermotoga maritima 3,4-dinitrophenol + D-glucose 6-phosphate
-
 ?
3,5-dichlorophenyl 6-phospho-beta-D-glucoside + H2O
-
 Thermotoga maritima 3,5-dichlorophenol + D-glucose 6-phosphate
-
 ?
3-nitrophenyl 6-phospho-beta-D-glucoside + H2O
-
 Thermotoga maritima 3-nitrophenol + D-glucose 6-phosphate
-
 ?
4-chloro-2-nitrophenyl 6-phospho-beta-D-glucoside + H2O
-
 Thermotoga maritima 4-chloro-2-nitrophenol + D-glucose 6-phosphate
-
 ?
4-cyanophenyl 6-phospho-beta-D-glucoside + H2O
-
 Thermotoga maritima 4-cyanophenol + D-glucose 6-phosphate
-
 ?
4-nitrophenyl 6-phospho-beta-D-glucoside + H2O
-
 Thermotoga maritima 4-nitrophenol + D-glucose 6-phosphate
-
 ?
4-tert-butylphenyl 6-phospho-beta-D-glucoside + H2O
-
 Thermotoga maritima 4-tert-butylphenol + D-glucose 6-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
6-phospho-beta-glucosidase
-
 Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.79
-
 4-cyanophenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima
0.99
-
 4-nitrophenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima
1.01
-
 4-cyanophenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima
1.15
-
 3,4-dinitrophenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima
1.21
-
 2-nitrophenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima
1.21
-
 4-tert-butylphenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima
1.69
-
 4-chloro-2-nitrophenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima
1.95
-
 2,5-dinitrophenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima
2.29
-
 2,4-dinitrophenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima
2.33
-
 3,5-dichlorophenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima
4.69
-
 3-nitrophenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima

Cofactor

Cofactor Comment Organism Structure
NAD+ enzyme is completely inactive in the absence of NAD+. Kd-value is 480 nM Thermotoga maritima