BRENDA - Enzyme Database show
show all sequences of 3.2.1.86

Structural and biochemical analysis of the asc operon encoding 6-phospho-beta-glucosidase in Pectobacterium carotovorum subsp. carotovorum LY34

An, C.L.; Lim, W.J.; Hong, S.Y.; Shin, E.C.; Kim, M.K.; Lee, J.R.; Park, S.R.; Woo, J.G.; Lim, Y.P.; Yun, H.D.; Res. Microbiol. 156, 145-153 (2005)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Pectobacterium carotovorum
Engineering
Amino acid exchange
Commentary
Organism
E182A
inactive mutant
Pectobacterium carotovorum
E374A
inactive mutant
Pectobacterium carotovorum
Inhibitors
Inhibitors
Commentary
Organism
Structure
Co2+
5 mM, 12% inhibition
Pectobacterium carotovorum
Cu2+
5 mM, 8% inhibition
Pectobacterium carotovorum
Hg2+
5 mM, complete inhibition
Pectobacterium carotovorum
Mn2+
5 mM, 14% inhibition
Pectobacterium carotovorum
Zn2+
5 mM, 13% inhibition
Pectobacterium carotovorum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.21
-
p-nitrophenyl-beta-D-glucopyranoside
pH 7.0, 40°C
Pectobacterium carotovorum
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
enhances activity, at 5 mM 11% increase in activity compared to activity without added ion
Pectobacterium carotovorum
Mg2+
enhances activity, maximal hydrolysis of p-nitrophenyl-beta-D-glucopyranoside at 5 mM, 7% increase in activity compared to activity without added ion
Pectobacterium carotovorum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
53000
-
x * 53000, SDS-PAGE
Pectobacterium carotovorum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pectobacterium carotovorum
-
subsp. carotovorum LY34
-
Purification (Commentary)
Commentary
Organism
-
Pectobacterium carotovorum
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
565
-
-
Pectobacterium carotovorum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-methylumbelliferyl-beta-D-glucoside + H2O
-
666900
Pectobacterium carotovorum
4-methylumbelliferone + D-glucose
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside + H2O
-
666900
Pectobacterium carotovorum
p-nitrophenol + D-glucose
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside-6-phosphate + H2O
-
666900
Pectobacterium carotovorum
p-nitrophenol + D-glucose 6-phosphate
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 53000, SDS-PAGE
Pectobacterium carotovorum
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
pH 7.0, hydrolysis of p-nitrophenyl-beta-D-glucopyranoside
Pectobacterium carotovorum
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
50
20°C: 55% of maximal activity, 50°C: 48% of maximal activity
Pectobacterium carotovorum
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
-
10 min, 39% loss of activity
Pectobacterium carotovorum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Pectobacterium carotovorum
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4
9
pH 4.0: about 60% of maximal activity, pH 9.0: about 30% of maximal activity
Pectobacterium carotovorum
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Pectobacterium carotovorum
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
E182A
inactive mutant
Pectobacterium carotovorum
E374A
inactive mutant
Pectobacterium carotovorum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Co2+
5 mM, 12% inhibition
Pectobacterium carotovorum
Cu2+
5 mM, 8% inhibition
Pectobacterium carotovorum
Hg2+
5 mM, complete inhibition
Pectobacterium carotovorum
Mn2+
5 mM, 14% inhibition
Pectobacterium carotovorum
Zn2+
5 mM, 13% inhibition
Pectobacterium carotovorum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.21
-
p-nitrophenyl-beta-D-glucopyranoside
pH 7.0, 40°C
Pectobacterium carotovorum
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
enhances activity, at 5 mM 11% increase in activity compared to activity without added ion
Pectobacterium carotovorum
Mg2+
enhances activity, maximal hydrolysis of p-nitrophenyl-beta-D-glucopyranoside at 5 mM, 7% increase in activity compared to activity without added ion
Pectobacterium carotovorum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
53000
-
x * 53000, SDS-PAGE
Pectobacterium carotovorum
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pectobacterium carotovorum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
565
-
-
Pectobacterium carotovorum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-methylumbelliferyl-beta-D-glucoside + H2O
-
666900
Pectobacterium carotovorum
4-methylumbelliferone + D-glucose
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside + H2O
-
666900
Pectobacterium carotovorum
p-nitrophenol + D-glucose
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside-6-phosphate + H2O
-
666900
Pectobacterium carotovorum
p-nitrophenol + D-glucose 6-phosphate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 53000, SDS-PAGE
Pectobacterium carotovorum
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
pH 7.0, hydrolysis of p-nitrophenyl-beta-D-glucopyranoside
Pectobacterium carotovorum
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
50
20°C: 55% of maximal activity, 50°C: 48% of maximal activity
Pectobacterium carotovorum
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
-
10 min, 39% loss of activity
Pectobacterium carotovorum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Pectobacterium carotovorum
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4
9
pH 4.0: about 60% of maximal activity, pH 9.0: about 30% of maximal activity
Pectobacterium carotovorum
Other publictions for EC 3.2.1.86
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
750522
Lansky
Structural basis for enzyme b ...
Geobacillus stearothermophilus, Geobacillus stearothermophilus T-1
FEBS J.
284
3931-3953
2017
-
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1
3
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1
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1
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1
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1
3
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1
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1
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1
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1
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-
751192
Yang
-
Coloring and bioinformatic an ...
Oenococcus oeni
J. Chin. Inst. Food Sci. Technol.
17
197-205
2017
-
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1
-
-
-
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1
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1
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1
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1
1
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1
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750546
Kwan
Chemoenzymatic synthesis of 6 ...
Streptococcus pyogenes, Streptococcus pyogenes M1
FEBS Lett.
590
461-468
2016
-
-
1
1
-
-
1
1
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-
-
-
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2
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1
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2
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1
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1
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1
1
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1
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2
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-
1
-
-
-
-
-
-
-
-
-
-
750733
Terra
Pneumococcal 6-phospho-beta-g ...
Streptococcus pneumoniae
Infect. Immun.
84
286-292
2016
-
-
1
-
-
-
-
1
1
-
-
2
-
4
-
-
1
-
-
-
1
-
2
1
-
-
-
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-
-
-
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-
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1
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1
1
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2
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1
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1
-
2
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
750963
Zangoui
Evolution of aromatic beta-gl ...
Escherichia coli
J. Bacteriol.
197
710-716
2015
-
-
-
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1
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1
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4
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1
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1
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4
-
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-
-
-
-
-
-
-
-
-
-
731055
Stepper
Structure and activity of the ...
Streptococcus pyogenes, Streptococcus pyogenes M1 GAS SF370
Acta Crystallogr. Sect. D
69
16-23
2013
-
-
1
1
-
1
1
2
-
-
-
-
-
5
-
-
1
-
-
-
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4
1
1
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1
1
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1
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1
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1
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1
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2
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1
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-
4
1
1
-
-
1
1
-
-
-
-
3
3
-
1
1
731178
Yang
Characterization of a novel me ...
uncultured organism
Appl. Environ. Microbiol.
79
2121-2127
2013
-
-
1
-
11
-
4
1
-
3
-
-
-
1
-
-
1
-
-
-
-
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2
-
1
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1
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1
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11
-
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4
-
1
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3
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1
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-
-
-
2
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
732101
Yu
Structural insights into the s ...
Streptococcus pneumoniae
J. Biol. Chem.
288
14949-14958
2013
-
-
1
1
13
-
-
6
-
-
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1
-
4
-
-
1
1
-
-
-
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3
-
1
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6
1
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-
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1
-
1
13
-
-
-
-
6
-
-
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1
-
-
-
1
-
-
-
-
3
-
1
-
-
6
1
-
-
-
-
2
2
-
6
6
732252
Honda
Purification and characterizat ...
Lactobacillus gasseri, Lactobacillus gasseri ATCC 33323
J. Gen. Appl. Microbiol.
58
11-17
2012
-
-
1
-
-
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-
2
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1
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6
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1
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8
1
2
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2
2
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2
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2
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1
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2
-
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-
8
1
2
-
-
2
2
-
-
-
-
1
2
-
2
2
715955
Capaldo
-
beta-Glucoside metabolism in O ...
Oenococcus oeni
J. Mol. Catal. B
69
27-34
2011
-
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1
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-
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1
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1
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1
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2
1
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1
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1
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1
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1
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1
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2
1
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1
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1
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-
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-
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-
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-
705622
Hall
Evolution and biochemistry of ...
Erwinia rhapontici, Erwinia rhapontici DSM 448
Mol. Biol. Evol.
26
2487-2497
2009
1
-
1
-
3
-
-
-
1
1
-
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-
4
-
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1
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2
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1
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1
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1
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3
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1
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1
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2
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1
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1
-
-
-
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-
678941
Vilei
beta-D-Glucoside utilization b ...
Mycoplasma mycoides
BMC Microbiol.
7
31
2007
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1
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5
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2
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681716
Honda
beta-Galactosidase, phospho-be ...
Lactobacillus acidophilus, Lactobacillus acidophilus JCM 1132T, Lactobacillus amylovorus, Lactobacillus casei, Lactobacillus crispatus, Lactobacillus fermentum, Lactobacillus gasseri, Lactobacillus johnsonii, Lactobacillus mucosae
Lett. Appl. Microbiol.
45
461-466
2007
-
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1
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14
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1
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1
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1
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1
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678190
Yip
Mechanistic analysis of the un ...
Thermotoga maritima
Biochemistry
45
571-580
2006
-
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11
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2
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10
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11
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1
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1
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11
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10
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11
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666041
Varrot
NAD+ and Metal-ion Dependent H ...
Thermotoga maritima
J. Mol. Biol.
346
423-435
2005
-
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1
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2
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2
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-
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-
666900
An
Structural and biochemical ana ...
Pectobacterium carotovorum
Res. Microbiol.
156
145-153
2005
-
-
1
-
2
-
5
1
-
2
1
-
-
4
-
-
1
-
-
-
1
-
3
1
1
1
1
-
1
1
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1
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2
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5
-
1
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2
1
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1
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1
-
3
1
1
1
1
-
1
1
-
-
-
-
-
-
-
-
655795
Yip
An unusual mechanism of glycos ...
Thermotoga maritima
J. Am. Chem. Soc.
126
8354-8355
2004
3
-
1
-
-
-
-
1
-
3
-
1
-
1
-
-
-
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-
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2
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1
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1
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1
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3
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1
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2
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1
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-
-
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-
-
-
656717
Weber
Expression of the phospho-beta ...
Lactobacillus delbrueckii
Microbiology
146
1941-1948
2000
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1
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1
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4
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1
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4
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136928
Thompson
Cellobiose-6-phosphate hydrola ...
Escherichia coli
J. Bacteriol.
181
7339-7345
1999
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7
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2
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2
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6
1
1
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7
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2
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6
1
1
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136927
Thompson
Phospho-beta-glucosidase from ...
Fusobacterium mortiferum
J. Bacteriol.
179
1636-1645
1997
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1
9
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3
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3
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1
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1
11
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1
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1
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1
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9
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3
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1
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1
11
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1
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1
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136929
Simons
Integration and gene replaceme ...
Lactococcus lactis
J. Bacteriol.
175
5168-5175
1993
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2
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1
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1
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136922
Schnetz
beta-Glucoside (bgl) operon of ...
Escherichia coli
J. Bacteriol.
169
2579-2590
1987
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5
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2
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2
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136923
Anderson
Phosphocellobiase ...
Klebsiella aerogenes
Methods Enzymol.
42 C
494-497
1975
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7
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1
1
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1
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1
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1
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8
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1
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7
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1
1
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1
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1
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8
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1
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136924
Wilson
The beta-glucoside system of E ...
Escherichia coli
J. Biol. Chem.
249
5586-5598
1974
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10
9
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4
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1
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1
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1
2
6
1
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2
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2
1
1
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10
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9
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4
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1
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1
2
6
1
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2
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2
1
1
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-
136926
Prasad
Genetic determination of the c ...
Escherichia coli
J. Bacteriol.
114
909-915
1973
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1
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3
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3
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136925
Palmer
Cellobiose metabolism in Aerob ...
Klebsiella aerogenes
J. Biol. Chem.
247
3420-3423
1972
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7
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1
1
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1
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1
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1
1
8
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1
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7
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1
1
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1
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1
1
8
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1
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