Literature summary for 3.2.1.86 extracted from

Varrot, A.; Yip, V.L.; Li, Y.; Rajan, S.S.; Yang, X.; Anderson, W.F.; Thompson, J.; Withers, S.G.; Davies, G.J.
NAD+ and Metal-ion Dependent Hydrolysis by Family 4 Glycosidases: Structural Insight into Specificity for Phospho-b-D-glucosides (2005), J. Mol. Biol., 346, 423-435.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging-drop vapor-diffusion method, crystal structure of the native selenomethionine-substituted enzyme (2.85 A resolution) and of the enzyme in complex with NAD+, Mn2+ and glucose 6-phosphate (2.55 A resolution)	Thermotoga maritima

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.041	-	p-nitrophenyl-beta-D-glucopyranoside 6-phosphate	pH 7.5, 50°C	Thermotoga maritima
0.206	-	p-nitrophenyl 6-phospho-beta-D-thioglucoside	pH 7.5, 50°C	Thermotoga maritima

Organism

Organism	UniProt	Comment	Textmining
Thermotoga maritima	Q9X108	recombinant	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
p-nitrophenyl 6-phospho-beta-D-thioglucoside + H2O	-	Thermotoga maritima	p-nitrophenol + 6-phospho-beta-D-thioglucose	-	?
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O	-	Thermotoga maritima	p-nitrophenol + beta-D-glucopyranose 6-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
BglT	-	Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.6	-	p-nitrophenyl 6-phospho-beta-D-thioglucoside	pH 7.5, 50°C	Thermotoga maritima
1.9	-	p-nitrophenyl-beta-D-glucopyranoside 6-phosphate	pH 7.5, 50°C	Thermotoga maritima

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Release 2023.1 (January 2023)