BRENDA - Enzyme Database show
show all sequences of 3.2.1.86

Cellobiose-6-phosphate hydrolase (CelF) of Escherichia coli: Characterization and Assignment to the unsual family 4 of glycosylhydrolases

Thompson, J.; Ruvinov, S.B.; Freedberg, D.I.; Hall, B.G.; J. Bacteriol. 181, 7339-7345 (1999)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.3
-
4-methyl-2-oxo-2H-chromen-7-yl 6-O-phosphono-beta-D-glucopyranoside
-
Escherichia coli
0.35
-
arbutin 6-phosphate
-
Escherichia coli
0.44
-
p-nitrophenyl-beta-D-glucopyranoside 6-P
-
Escherichia coli
0.44
-
Salicin 6-phosphate
-
Escherichia coli
1.3
-
cellobiose 6-phosphate
-
Escherichia coli
2.22
-
methyl beta-D-glucoside 6-phosphate
-
Escherichia coli
12.5
-
6-O-(6-O-phosphono-beta-D-glucopyranosyl)-beta-D-glucopyranose
-
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
21000
-
gel filtration
Escherichia coli
50000
-
SDS-PAGE
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
P17411
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
arbutin 6-phosphate + H2O
-
136928
Escherichia coli
hydroquinone + D-glucose 6-phosphate
-
-
-
?
cellobiose 6-phosphate + H2O
-
136928
Escherichia coli
D-glucose 6-phosphate + D-glucose
-
-
-
-
gentiobiose 6-phosphate + H2O
-
136928
Escherichia coli
D-glucose 6-phosphate + D-glucose
-
-
-
-
methyl beta-glucoside 6-phosphate + H2O
-
136928
Escherichia coli
?
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
-
136928
Escherichia coli
p-nitrophenol + D-glucopyranose 6-phosphate
-
-
-
?
salicin 6-phosphate + H2O
-
136928
Escherichia coli
2-hydroxymethylphenol + D-glucose 6-phosphate
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
4 * 50000
Escherichia coli
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
34
38
-
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.3
-
4-methyl-2-oxo-2H-chromen-7-yl 6-O-phosphono-beta-D-glucopyranoside
-
Escherichia coli
0.35
-
arbutin 6-phosphate
-
Escherichia coli
0.44
-
p-nitrophenyl-beta-D-glucopyranoside 6-P
-
Escherichia coli
0.44
-
Salicin 6-phosphate
-
Escherichia coli
1.3
-
cellobiose 6-phosphate
-
Escherichia coli
2.22
-
methyl beta-D-glucoside 6-phosphate
-
Escherichia coli
12.5
-
6-O-(6-O-phosphono-beta-D-glucopyranosyl)-beta-D-glucopyranose
-
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
21000
-
gel filtration
Escherichia coli
50000
-
SDS-PAGE
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
arbutin 6-phosphate + H2O
-
136928
Escherichia coli
hydroquinone + D-glucose 6-phosphate
-
-
-
?
cellobiose 6-phosphate + H2O
-
136928
Escherichia coli
D-glucose 6-phosphate + D-glucose
-
-
-
-
gentiobiose 6-phosphate + H2O
-
136928
Escherichia coli
D-glucose 6-phosphate + D-glucose
-
-
-
-
methyl beta-glucoside 6-phosphate + H2O
-
136928
Escherichia coli
?
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
-
136928
Escherichia coli
p-nitrophenol + D-glucopyranose 6-phosphate
-
-
-
?
salicin 6-phosphate + H2O
-
136928
Escherichia coli
2-hydroxymethylphenol + D-glucose 6-phosphate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 50000
Escherichia coli
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
34
38
-
Escherichia coli
Other publictions for EC 3.2.1.86
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
750522
Lansky
Structural basis for enzyme b ...
Geobacillus stearothermophilus, Geobacillus stearothermophilus T-1
FEBS J.
284
3931-3953
2017
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1
3
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1
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3
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1
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1
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1
3
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1
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1
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2
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
751192
Yang
-
Coloring and bioinformatic an ...
Oenococcus oeni
J. Chin. Inst. Food Sci. Technol.
17
197-205
2017
-
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1
-
-
-
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-
1
-
-
1
-
1
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1
1
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1
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1
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1
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1
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1
1
-
-
-
-
-
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-
1
-
-
-
-
-
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750546
Kwan
Chemoenzymatic synthesis of 6 ...
Streptococcus pyogenes, Streptococcus pyogenes M1
FEBS Lett.
590
461-468
2016
-
-
1
1
-
-
1
1
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-
-
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2
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1
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2
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1
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1
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1
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1
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1
1
1
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1
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-
2
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1
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-
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-
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-
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-
750733
Terra
Pneumococcal 6-phospho-beta-g ...
Streptococcus pneumoniae
Infect. Immun.
84
286-292
2016
-
-
1
-
-
-
-
1
1
-
-
2
-
4
-
-
1
-
-
-
1
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2
1
-
-
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-
-
-
-
-
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1
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1
1
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2
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1
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-
1
-
2
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
750963
Zangoui
Evolution of aromatic beta-gl ...
Escherichia coli
J. Bacteriol.
197
710-716
2015
-
-
-
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1
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-
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1
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1
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-
-
-
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4
-
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1
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1
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-
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4
-
-
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-
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-
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-
731055
Stepper
Structure and activity of the ...
Streptococcus pyogenes, Streptococcus pyogenes M1 GAS SF370
Acta Crystallogr. Sect. D
69
16-23
2013
-
-
1
1
-
1
1
2
-
-
-
-
-
5
-
-
1
-
-
-
-
-
4
1
1
-
-
1
1
-
-
-
-
-
-
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1
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1
-
1
-
1
-
2
-
-
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-
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-
-
1
-
-
-
-
4
1
1
-
-
1
1
-
-
-
-
3
3
-
1
1
731178
Yang
Characterization of a novel me ...
uncultured organism
Appl. Environ. Microbiol.
79
2121-2127
2013
-
-
1
-
11
-
4
1
-
3
-
-
-
1
-
-
1
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
11
-
-
4
-
1
-
3
-
-
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
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-
-
-
2
2
-
-
-
732101
Yu
Structural insights into the s ...
Streptococcus pneumoniae
J. Biol. Chem.
288
14949-14958
2013
-
-
1
1
13
-
-
6
-
-
-
1
-
4
-
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1
1
-
-
-
-
3
-
1
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-
6
1
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-
-
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1
-
1
13
-
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-
-
6
-
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1
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-
1
-
-
-
-
3
-
1
-
-
6
1
-
-
-
-
2
2
-
6
6
732252
Honda
Purification and characterizat ...
Lactobacillus gasseri, Lactobacillus gasseri ATCC 33323
J. Gen. Appl. Microbiol.
58
11-17
2012
-
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1
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-
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-
2
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1
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6
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1
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8
1
2
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2
2
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-
2
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2
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1
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2
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8
1
2
-
-
2
2
-
-
-
-
1
2
-
2
2
715955
Capaldo
-
beta-Glucoside metabolism in O ...
Oenococcus oeni
J. Mol. Catal. B
69
27-34
2011
-
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1
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-
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1
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1
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1
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2
1
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1
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1
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1
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1
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1
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2
1
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1
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1
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-
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-
-
-
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-
705622
Hall
Evolution and biochemistry of ...
Erwinia rhapontici, Erwinia rhapontici DSM 448
Mol. Biol. Evol.
26
2487-2497
2009
1
-
1
-
3
-
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-
1
1
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-
4
-
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1
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-
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2
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1
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1
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1
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3
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1
1
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1
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2
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1
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1
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678941
Vilei
beta-D-Glucoside utilization b ...
Mycoplasma mycoides
BMC Microbiol.
7
31
2007
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1
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5
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2
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681716
Honda
beta-Galactosidase, phospho-be ...
Lactobacillus acidophilus, Lactobacillus acidophilus JCM 1132T, Lactobacillus amylovorus, Lactobacillus casei, Lactobacillus crispatus, Lactobacillus fermentum, Lactobacillus gasseri, Lactobacillus johnsonii, Lactobacillus mucosae
Lett. Appl. Microbiol.
45
461-466
2007
-
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1
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14
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1
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1
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1
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1
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678190
Yip
Mechanistic analysis of the un ...
Thermotoga maritima
Biochemistry
45
571-580
2006
-
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11
-
-
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2
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-
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10
-
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11
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1
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1
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11
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-
10
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11
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666041
Varrot
NAD+ and Metal-ion Dependent H ...
Thermotoga maritima
J. Mol. Biol.
346
423-435
2005
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1
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2
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4
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2
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666900
An
Structural and biochemical ana ...
Pectobacterium carotovorum
Res. Microbiol.
156
145-153
2005
-
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1
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2
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5
1
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2
1
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4
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1
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1
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3
1
1
1
1
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1
1
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1
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2
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5
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1
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2
1
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1
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1
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3
1
1
1
1
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1
1
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655795
Yip
An unusual mechanism of glycos ...
Thermotoga maritima
J. Am. Chem. Soc.
126
8354-8355
2004
3
-
1
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1
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3
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1
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1
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1
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1
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2
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1
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656717
Weber
Expression of the phospho-beta ...
Lactobacillus delbrueckii
Microbiology
146
1941-1948
2000
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1
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1
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4
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1
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4
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136928
Thompson
Cellobiose-6-phosphate hydrola ...
Escherichia coli
J. Bacteriol.
181
7339-7345
1999
-
-
-
-
-
-
-
7
-
-
2
-
-
2
-
-
-
-
-
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6
1
1
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-
-
-
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7
-
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2
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6
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
136927
Thompson
Phospho-beta-glucosidase from ...
Fusobacterium mortiferum
J. Bacteriol.
179
1636-1645
1997
-
-
-
-
-
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1
9
-
-
3
-
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3
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1
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1
11
-
1
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1
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1
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9
-
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3
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1
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1
11
-
1
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1
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-
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-
136929
Simons
Integration and gene replaceme ...
Lactococcus lactis
J. Bacteriol.
175
5168-5175
1993
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2
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1
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136922
Schnetz
beta-Glucoside (bgl) operon of ...
Escherichia coli
J. Bacteriol.
169
2579-2590
1987
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5
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2
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-
-
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-
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-
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2
-
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136923
Anderson
Phosphocellobiase ...
Klebsiella aerogenes
Methods Enzymol.
42 C
494-497
1975
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7
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1
1
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1
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1
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1
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8
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1
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7
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1
1
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1
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1
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8
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1
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136924
Wilson
The beta-glucoside system of E ...
Escherichia coli
J. Biol. Chem.
249
5586-5598
1974
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10
9
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4
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1
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1
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1
2
6
1
-
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2
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2
1
1
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10
-
9
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4
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1
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1
2
6
1
-
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2
-
2
1
1
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136926
Prasad
Genetic determination of the c ...
Escherichia coli
J. Bacteriol.
114
909-915
1973
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1
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3
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3
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136925
Palmer
Cellobiose metabolism in Aerob ...
Klebsiella aerogenes
J. Biol. Chem.
247
3420-3423
1972
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7
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1
1
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1
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1
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1
1
8
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1
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7
-
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1
1
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1
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1
1
8
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1
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