BRENDA - Enzyme Database show
show all sequences of 3.2.1.86

Phospho-beta-glucosidase from Fusobacterium mortiferum: Purification, clonig, and inactivation by 6-phosphoglucono-sigma-lactone

Thompson, J.; Robrish, S.A.; Bouma, C.L.; Freedberg, D.I.; Folk, J.E.; J. Bacteriol. 179, 1636-1645 (1997)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
D-glucono-delta-lactone
-
Fusobacterium mortiferum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0092
-
4-methylumbelliferyl-beta-D-glucopyranoside 6-phosphate
-
Fusobacterium mortiferum
0.0291
-
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate
-
Fusobacterium mortiferum
0.0875
-
laminaribiose 6-phosphate
-
Fusobacterium mortiferum
0.0886
-
Salicin 6-phosphate
-
Fusobacterium mortiferum
0.0977
-
arbutin 6-phosphate
-
Fusobacterium mortiferum
0.1212
-
sophorose 6-phosphate
-
Fusobacterium mortiferum
0.1384
-
methyl-beta-D-glucoside 6-phosphate
-
Fusobacterium mortiferum
0.1823
-
cellobiose 6-phosphate
-
Fusobacterium mortiferum
0.3355
-
gentiobiose 6-phosphate
-
Fusobacterium mortiferum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
53000
-
SDS-PAGE
Fusobacterium mortiferum
53560
-
MALDI-MS
Fusobacterium mortiferum
54000
-
gel filtration chromatography
Fusobacterium mortiferum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Fusobacterium mortiferum
-
-
-
Purification (Commentary)
Commentary
Organism
23 fold
Fusobacterium mortiferum
Storage Stability
Storage Stability
Organism
-20°C, 6 months, no significant decline in activity
Fusobacterium mortiferum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-methylumbelliferyl-beta-D-glucopyranoside 6-phosphate + H2O
-
136927
Fusobacterium mortiferum
4-methylumbelliferone + D-glucose 6-phosphate
-
-
-
?
arbutin 6-phosphate + H2O
-
136927
Fusobacterium mortiferum
hydroquinone + D-glucose 6-phosphate
-
-
-
?
cellobiose 6-phosphate + H2O
-
136927
Fusobacterium mortiferum
D-glucose 6-phosphate + D-glucose
-
-
-
-
esculin 6-phosphate + H2O
-
136927
Fusobacterium mortiferum
?
-
-
-
?
gentiobiose 6-phosphate + H2O
-
136927
Fusobacterium mortiferum
D-glucose 6-phosphate + D-glucose
-
-
-
?
laminaribiose 6-phosphate + H2O
-
136927
Fusobacterium mortiferum
D-glucose 6-phosphate + D-glucose
-
-
-
?
methyl beta-D-glucoside monophosphate + H2O
-
136927
Fusobacterium mortiferum
?
-
-
-
-
methyl beta-glucoside 6-phosphate + H2O
-
136927
Fusobacterium mortiferum
?
-
-
-
-
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
-
136927
Fusobacterium mortiferum
p-nitrophenol + D-glucopyranose 6-phosphate
-
-
-
?
salicin 6-phosphate + H2O
-
136927
Fusobacterium mortiferum
2-hydroxymethylphenol + D-glucose 6-phosphate
-
-
-
-
sophorose 6-phosphate + H2O
-
136927
Fusobacterium mortiferum
D-glucose 6-phosphate + D-glucose
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
40
-
Fusobacterium mortiferum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
7
-
Fusobacterium mortiferum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
D-glucono-delta-lactone
-
Fusobacterium mortiferum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0092
-
4-methylumbelliferyl-beta-D-glucopyranoside 6-phosphate
-
Fusobacterium mortiferum
0.0291
-
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate
-
Fusobacterium mortiferum
0.0875
-
laminaribiose 6-phosphate
-
Fusobacterium mortiferum
0.0886
-
Salicin 6-phosphate
-
Fusobacterium mortiferum
0.0977
-
arbutin 6-phosphate
-
Fusobacterium mortiferum
0.1212
-
sophorose 6-phosphate
-
Fusobacterium mortiferum
0.1384
-
methyl-beta-D-glucoside 6-phosphate
-
Fusobacterium mortiferum
0.1823
-
cellobiose 6-phosphate
-
Fusobacterium mortiferum
0.3355
-
gentiobiose 6-phosphate
-
Fusobacterium mortiferum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
53000
-
SDS-PAGE
Fusobacterium mortiferum
53560
-
MALDI-MS
Fusobacterium mortiferum
54000
-
gel filtration chromatography
Fusobacterium mortiferum
Purification (Commentary) (protein specific)
Commentary
Organism
23 fold
Fusobacterium mortiferum
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, 6 months, no significant decline in activity
Fusobacterium mortiferum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-methylumbelliferyl-beta-D-glucopyranoside 6-phosphate + H2O
-
136927
Fusobacterium mortiferum
4-methylumbelliferone + D-glucose 6-phosphate
-
-
-
?
arbutin 6-phosphate + H2O
-
136927
Fusobacterium mortiferum
hydroquinone + D-glucose 6-phosphate
-
-
-
?
cellobiose 6-phosphate + H2O
-
136927
Fusobacterium mortiferum
D-glucose 6-phosphate + D-glucose
-
-
-
-
esculin 6-phosphate + H2O
-
136927
Fusobacterium mortiferum
?
-
-
-
?
gentiobiose 6-phosphate + H2O
-
136927
Fusobacterium mortiferum
D-glucose 6-phosphate + D-glucose
-
-
-
?
laminaribiose 6-phosphate + H2O
-
136927
Fusobacterium mortiferum
D-glucose 6-phosphate + D-glucose
-
-
-
?
methyl beta-D-glucoside monophosphate + H2O
-
136927
Fusobacterium mortiferum
?
-
-
-
-
methyl beta-glucoside 6-phosphate + H2O
-
136927
Fusobacterium mortiferum
?
-
-
-
-
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
-
136927
Fusobacterium mortiferum
p-nitrophenol + D-glucopyranose 6-phosphate
-
-
-
?
salicin 6-phosphate + H2O
-
136927
Fusobacterium mortiferum
2-hydroxymethylphenol + D-glucose 6-phosphate
-
-
-
-
sophorose 6-phosphate + H2O
-
136927
Fusobacterium mortiferum
D-glucose 6-phosphate + D-glucose
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
40
-
Fusobacterium mortiferum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
7
-
Fusobacterium mortiferum
Other publictions for EC 3.2.1.86
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
750522
Lansky
Structural basis for enzyme b ...
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284
3931-3953
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-
-
-
1
3
-
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1
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3
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1
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1
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3
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1
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2
1
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-
-
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1
-
-
1
-
-
751192
Yang
-
Coloring and bioinformatic an ...
Oenococcus oeni
J. Chin. Inst. Food Sci. Technol.
17
197-205
2017
-
-
1
-
-
-
-
-
1
-
-
1
-
1
-
-
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-
-
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1
1
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1
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1
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1
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1
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1
1
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-
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-
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-
1
-
-
-
-
-
-
750546
Kwan
Chemoenzymatic synthesis of 6 ...
Streptococcus pyogenes, Streptococcus pyogenes M1
FEBS Lett.
590
461-468
2016
-
-
1
1
-
-
1
1
-
-
-
-
-
2
-
-
1
-
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2
-
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1
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1
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1
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1
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1
1
1
-
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-
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1
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
750733
Terra
Pneumococcal 6-phospho-beta-g ...
Streptococcus pneumoniae
Infect. Immun.
84
286-292
2016
-
-
1
-
-
-
-
1
1
-
-
2
-
4
-
-
1
-
-
-
1
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2
1
-
-
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-
-
-
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-
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1
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1
1
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2
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1
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1
-
2
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
750963
Zangoui
Evolution of aromatic beta-gl ...
Escherichia coli
J. Bacteriol.
197
710-716
2015
-
-
-
-
1
-
-
-
-
-
-
1
-
1
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-
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4
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1
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1
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-
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-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
731055
Stepper
Structure and activity of the ...
Streptococcus pyogenes, Streptococcus pyogenes M1 GAS SF370
Acta Crystallogr. Sect. D
69
16-23
2013
-
-
1
1
-
1
1
2
-
-
-
-
-
5
-
-
1
-
-
-
-
-
4
1
1
-
-
1
1
-
-
-
-
-
-
-
-
1
-
1
-
1
-
1
-
2
-
-
-
-
-
-
-
1
-
-
-
-
4
1
1
-
-
1
1
-
-
-
-
3
3
-
1
1
731178
Yang
Characterization of a novel me ...
uncultured organism
Appl. Environ. Microbiol.
79
2121-2127
2013
-
-
1
-
11
-
4
1
-
3
-
-
-
1
-
-
1
-
-
-
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2
-
1
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1
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1
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-
11
-
-
4
-
1
-
3
-
-
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
732101
Yu
Structural insights into the s ...
Streptococcus pneumoniae
J. Biol. Chem.
288
14949-14958
2013
-
-
1
1
13
-
-
6
-
-
-
1
-
4
-
-
1
1
-
-
-
-
3
-
1
-
-
6
1
-
-
-
-
-
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1
-
1
13
-
-
-
-
6
-
-
-
1
-
-
-
1
-
-
-
-
3
-
1
-
-
6
1
-
-
-
-
2
2
-
6
6
732252
Honda
Purification and characterizat ...
Lactobacillus gasseri, Lactobacillus gasseri ATCC 33323
J. Gen. Appl. Microbiol.
58
11-17
2012
-
-
1
-
-
-
-
2
-
-
1
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6
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1
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8
1
2
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2
2
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2
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2
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1
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2
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8
1
2
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2
2
-
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1
2
-
2
2
715955
Capaldo
-
beta-Glucoside metabolism in O ...
Oenococcus oeni
J. Mol. Catal. B
69
27-34
2011
-
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1
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1
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1
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1
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1
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1
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1
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1
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1
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2
1
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1
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1
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-
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-
-
-
705622
Hall
Evolution and biochemistry of ...
Erwinia rhapontici, Erwinia rhapontici DSM 448
Mol. Biol. Evol.
26
2487-2497
2009
1
-
1
-
3
-
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1
1
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-
-
4
-
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1
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2
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1
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1
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1
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1
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2
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1
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1
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678941
Vilei
beta-D-Glucoside utilization b ...
Mycoplasma mycoides
BMC Microbiol.
7
31
2007
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1
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5
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2
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681716
Honda
beta-Galactosidase, phospho-be ...
Lactobacillus acidophilus, Lactobacillus acidophilus JCM 1132T, Lactobacillus amylovorus, Lactobacillus casei, Lactobacillus crispatus, Lactobacillus fermentum, Lactobacillus gasseri, Lactobacillus johnsonii, Lactobacillus mucosae
Lett. Appl. Microbiol.
45
461-466
2007
-
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1
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14
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1
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1
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678190
Yip
Mechanistic analysis of the un ...
Thermotoga maritima
Biochemistry
45
571-580
2006
-
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11
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2
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10
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11
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1
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1
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11
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10
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11
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666041
Varrot
NAD+ and Metal-ion Dependent H ...
Thermotoga maritima
J. Mol. Biol.
346
423-435
2005
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1
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2
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4
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2
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2
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666900
An
Structural and biochemical ana ...
Pectobacterium carotovorum
Res. Microbiol.
156
145-153
2005
-
-
1
-
2
-
5
1
-
2
1
-
-
4
-
-
1
-
-
-
1
-
3
1
1
1
1
-
1
1
-
-
-
-
-
-
-
1
-
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2
-
-
5
-
1
-
2
1
-
-
-
-
1
-
-
1
-
3
1
1
1
1
-
1
1
-
-
-
-
-
-
-
-
655795
Yip
An unusual mechanism of glycos ...
Thermotoga maritima
J. Am. Chem. Soc.
126
8354-8355
2004
3
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1
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1
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3
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1
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1
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2
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1
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656717
Weber
Expression of the phospho-beta ...
Lactobacillus delbrueckii
Microbiology
146
1941-1948
2000
-
-
1
-
-
-
-
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-
1
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-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
136928
Thompson
Cellobiose-6-phosphate hydrola ...
Escherichia coli
J. Bacteriol.
181
7339-7345
1999
-
-
-
-
-
-
-
7
-
-
2
-
-
2
-
-
-
-
-
-
-
-
6
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
2
-
-
-
-
-
-
-
-
-
6
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
136927
Thompson
Phospho-beta-glucosidase from ...
Fusobacterium mortiferum
J. Bacteriol.
179
1636-1645
1997
-
-
-
-
-
-
1
9
-
-
3
-
-
3
-
-
1
-
-
-
-
1
11
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
9
-
-
3
-
-
-
-
1
-
-
-
1
11
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
136929
Simons
Integration and gene replaceme ...
Lactococcus lactis
J. Bacteriol.
175
5168-5175
1993
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-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
136922
Schnetz
beta-Glucoside (bgl) operon of ...
Escherichia coli
J. Bacteriol.
169
2579-2590
1987
-
-
-
-
-
-
-
-
-
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-
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
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-
-
-
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-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
136923
Anderson
Phosphocellobiase ...
Klebsiella aerogenes
Methods Enzymol.
42 C
494-497
1975
-
-
-
-
-
-
-
7
-
-
1
1
-
1
-
-
1
-
-
-
1
-
8
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
1
1
-
-
-
1
-
-
1
-
8
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
136924
Wilson
The beta-glucoside system of E ...
Escherichia coli
J. Biol. Chem.
249
5586-5598
1974
-
-
-
-
-
-
10
9
-
-
4
-
-
1
-
-
1
-
-
-
1
2
6
1
-
-
2
-
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
10
-
9
-
-
4
-
-
-
-
1
-
-
1
2
6
1
-
-
2
-
2
1
1
-
-
-
-
-
-
-
136926
Prasad
Genetic determination of the c ...
Escherichia coli
J. Bacteriol.
114
909-915
1973
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-
-
-
-
-
-
-
-
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-
1
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-
-
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-
-
3
-
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-
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-
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-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
136925
Palmer
Cellobiose metabolism in Aerob ...
Klebsiella aerogenes
J. Biol. Chem.
247
3420-3423
1972
-
-
-
-
-
-
-
7
-
-
1
1
-
1
-
-
1
-
-
-
1
1
8
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
1
1
-
-
-
1
-
-
1
1
8
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-