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Literature summary for 3.2.1.85 extracted from

  • Aleksandrzak-Piekarczyk, T.; Kok, J.; Renault, P.; Bardowski, J.
    Alternative lactose catabolic pathway in Lactococcus lactis IL1403 (2005), Appl. Environ. Microbiol., 71, 6060-6069.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.85

Cloned(Commentary)

Cloned (Comment) Organism
subcloning in Escherichia coli, expression of wild-type and mutant enzymes in Lactococcus lactis strain IBB50 Lactococcus lactis

Protein Variants

Protein Variants Comment Organism
additional information construction of a disruption mutant of gene bglS, encoding phospho-beta-glucosidase, in phospho-beta-galactosidase-deficient strain IL1403, genetic organization and chromosomal localizations in mutant strain IL1403 showig the beta-galactosidase-negative phenotype Lactococcus lactis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
lactose + H2O Lactococcus lactis existence of an alternative lactose catabolic pathway in the enzyme-deficient mutant strain IL1403 by phospho-beta-galactosidase activity exhibited by BglS, a phospho-beta-glucosidase, the lactose hydrolysis is catalyzed by both phospho-beta-galactosidase and phospho-beta-glucosidase, the latter being the main enzyme in the pathway D-glucose + D-galactose
-
 ?
additional information Lactococcus lactis physiologic regulation of the pathway, overview ?
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 ?

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
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 subsp. lactis, wild-type strains and enzyme-deficient mutant strain IL1403, overview
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Source Tissue

Source Tissue Comment Organism Textmining
additional information growth condition requirements of mutant strains Lactococcus lactis
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactopyranoside 6-phosphate + H2O
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 Lactococcus lactis 2-nitrophenyl beta-D-galactopyranose + phosphate
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 ?
lactose + H2O
-
 Lactococcus lactis D-glucose + D-galactose
-
 ?
lactose + H2O existence of an alternative lactose catabolic pathway in the enzyme-deficient mutant strain IL1403 by phospho-beta-galactosidase activity exhibited by BglS, a phospho-beta-glucosidase, the lactose hydrolysis is catalyzed by both phospho-beta-galactosidase and phospho-beta-glucosidase, the latter being the main enzyme in the pathway Lactococcus lactis D-glucose + D-galactose
-
 ?
additional information physiologic regulation of the pathway, overview Lactococcus lactis ?
-
 ?

Synonyms

Synonyms Comment Organism
P-beta-galactosidase
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 Lactococcus lactis
phospho-beta-galactosidase
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 Lactococcus lactis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Lactococcus lactis