Literature summary for 3.2.1.85 extracted from

Hengstenberg, W.; Morse, M.L.
6-phospho-beta-D-Galactosidase (1975), Methods Enzymol., 42, 491-494.

Search for more literature for 3.2.1.85

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.6	3	o-nitrophenyl-beta-D-galactopyranoside 6-phosphate	-	Staphylococcus aureus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cu2+	-	Staphylococcus aureus
Hg2+	-	Staphylococcus aureus
Mn2+	50% inactivation to 10 mM	Staphylococcus aureus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
46000	53000	gel filtration, SDS-PAGE	Staphylococcus aureus
50000	-	1 * 50000, SDS-PAGE	Staphylococcus aureus

Organism

Organism	UniProt	Comment	Textmining
Staphylococcus aureus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Staphylococcus aureus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
38	47	at 30°C	Staphylococcus aureus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O	-	Staphylococcus aureus	o-nitrophenol + beta-D-galactose-6-phosphate	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 50000, SDS-PAGE	Staphylococcus aureus

Synonyms

Synonyms	Comment	Organism
6-phospho-beta-D-galactosidase	-	Staphylococcus aureus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
55	-	50% of activity after 5 min in Tris-buffer	Staphylococcus aureus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Staphylococcus aureus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)