Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 102.1% activity at 10 mM | Pseudoalteromonas porphyrae |
Cloned (Comment) | Organism |
---|---|
expressed in Brevibacillus choshinensis | Pseudoalteromonas porphyrae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 2.1% residual activity at 50 mM | Pseudoalteromonas porphyrae | |
EDTA | 97.4% residual activity at 10 mM | Pseudoalteromonas porphyrae | |
EGTA | 87.8% residual activity at 10 mM | Pseudoalteromonas porphyrae | |
Fe2+ | 8.1% residual activity at 50 mM | Pseudoalteromonas porphyrae | |
Fe3+ | 9.2% residual activity at 50 mM | Pseudoalteromonas porphyrae | |
Hg2+ | 0.3% residual activity at 50 mM | Pseudoalteromonas porphyrae | |
Mn2+ | 53.5% residual activity at 50 mM | Pseudoalteromonas porphyrae | |
phenylmethylsulfonyl fluoride | 94.3% residual activity at 10 mM | Pseudoalteromonas porphyrae | |
SDS | 1.8% residual activity at 10 mM | Pseudoalteromonas porphyrae | |
Zn2+ | 8.3% residual activity at 50 mM | Pseudoalteromonas porphyrae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 100 mM Ca2+ enhances the activity of the enzyme by 38% | Pseudoalteromonas porphyrae | |
K+ | 100 mM K+ enhances the activity of the enzyme by 3% | Pseudoalteromonas porphyrae | |
Mg2+ | 100 mM Mg2+ enhances the activity of the enzyme by 97.5% | Pseudoalteromonas porphyrae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
kappa-carrageen + H2O | Pseudoalteromonas porphyrae | - |
kappa-neocarraoctaose + kappa-neocarrahexaose + kappa-neocarratetraose + kappa-neocarrabiose | - |
? | |
kappa-carrageen + H2O | Pseudoalteromonas porphyrae LL1 | - |
kappa-neocarraoctaose + kappa-neocarrahexaose + kappa-neocarratetraose + kappa-neocarrabiose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudoalteromonas porphyrae | - |
- |
- |
Pseudoalteromonas porphyrae LL1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His Trap column chromatography and Sephacryl S-100 gel filtration | Pseudoalteromonas porphyrae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
kappa-carrageen + H2O | - |
Pseudoalteromonas porphyrae | kappa-neocarraoctaose + kappa-neocarrahexaose + kappa-neocarratetraose + kappa-neocarrabiose | - |
? | |
kappa-carrageen + H2O | - |
Pseudoalteromonas porphyrae LL1 | kappa-neocarraoctaose + kappa-neocarrahexaose + kappa-neocarratetraose + kappa-neocarrabiose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000, SDS-PAGE | Pseudoalteromonas porphyrae |
? | x * 31500, calculated from amino acid sequence | Pseudoalteromonas porphyrae |
Synonyms | Comment | Organism |
---|---|---|
CGK | - |
Pseudoalteromonas porphyrae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Pseudoalteromonas porphyrae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 45 | the enzyme shows more than 70% from 30 to 45°C | Pseudoalteromonas porphyrae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 50 | over 80% of the total activity is retained after 60 min at 40°C. When the incubation temperature rises above 45°C, the activity of the enzyme decreases rapidly. Furthermore, the enzyme is almost inactivated at 50°C within 60 min | Pseudoalteromonas porphyrae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pseudoalteromonas porphyrae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | more than 50% activity between pH 6.0 and 9.0 | Pseudoalteromonas porphyrae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | the enzyme is considerably stable from pH 7.0 to 9.0, where its activity declines by less than 10% | Pseudoalteromonas porphyrae |