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Literature summary for 3.2.1.83 extracted from

  • Li, J.; Ni, H.; Cai, H.; Zhu, Y.; Xiao, A.
    Cloning, expression, and enzymatic properties of kappa-carrageenase from Pseudoalteromonas carrageenovora (2016), Mod. Food Sci. Technol., 32, 72-77 .
No PubMed abstract available
Search for more literature for 3.2.1.83

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pseudoalteromonas carrageenovora

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ the enzyme activity is inhibited by 1 mM Ba2+ Pseudoalteromonas carrageenovora
Ca2+ the enzyme activity is inhibited by 1 mM Ca2+ Pseudoalteromonas carrageenovora
Cd2+ the enzyme activity is inhibited by 1 mM Cd2+ Pseudoalteromonas carrageenovora
Fe2+ the enzyme activity is inhibited by 1 mM Fe2+ Pseudoalteromonas carrageenovora
K+ the enzyme activity is inhibited by high concentrations of K+ Pseudoalteromonas carrageenovora
Mg2+ the enzyme activity is inhibited by 1 mM Mg2+ Pseudoalteromonas carrageenovora
Na+ the enzyme activity is inhibited by high concentrations of Na+ Pseudoalteromonas carrageenovora

Metals/Ions

Metals/Ions Comment Organism Structure
2-mercaptoethanol the enzyme activity is significantly promoted at 1 mM 2-mercaptoethanol Pseudoalteromonas carrageenovora
dithiothreitol the enzyme activity is significantly promoted at 1 mM dithiothreitol Pseudoalteromonas carrageenovora
EDTA the enzyme activity is significantly promoted at 1 mM EDTA Pseudoalteromonas carrageenovora
Fe3+ the enzyme activity is significantly promoted at 1 mM Fe3+ Pseudoalteromonas carrageenovora
K+ the enzyme activity is significantly promoted at low concentrations of K+ Pseudoalteromonas carrageenovora
Na+ the enzyme activity is significantly promoted at low concentrations of Na+ Pseudoalteromonas carrageenovora
SDS the enzyme activity is significantly promoted at 1 mM SDS Pseudoalteromonas carrageenovora
Sn2+ the enzyme activity is significantly promoted at 1 mM Sn2+ Pseudoalteromonas carrageenovora
Triton X-100 the enzyme activity is significantly promoted at 1 mM Triton X-100 Pseudoalteromonas carrageenovora
Tween-20 the enzyme activity is significantly promoted at 1 mM Tween 20 Pseudoalteromonas carrageenovora
Tween-80 the enzyme activity is significantly promoted at 1 mM Tween 80 Pseudoalteromonas carrageenovora
Urea the enzyme activity is significantly promoted at 1 mM urea Pseudoalteromonas carrageenovora

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
kappa-carrageen + H2O Pseudoalteromonas carrageenovora specific substrate ?
-
 ?
kappa-carrageen + H2O Pseudoalteromonas carrageenovora ASY5 specific substrate ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudoalteromonas carrageenovora
-
-
-
Pseudoalteromonas carrageenovora ASY5
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
kappa-carrageen + H2O specific substrate Pseudoalteromonas carrageenovora ?
-
 ?
kappa-carrageen + H2O specific substrate Pseudoalteromonas carrageenovora ASY5 ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Pseudoalteromonas carrageenovora

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 45 the enzyme retains more than 95% and 60% of the maximum enzymatic activity after 1 h of incubation at 40°C and below 45°C, respectively Pseudoalteromonas carrageenovora

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Pseudoalteromonas carrageenovora

pH Stability

pH Stability pH Stability Maximum Comment Organism
8 10 the enzyme retains more than 80% of the maximum enzymatic activity after 30 min of incubation at a pH range of 8.0-10.0 Pseudoalteromonas carrageenovora