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Literature summary for 3.2.1.83 extracted from
- Expression and characterization of a kappa-carrageenase from marine bacterium Wenyingzhuangia aestuarii OF219 A biotechnological tool for the depolymerization of kappa-carrageenan (2018), Int. J. Biol. Macromol., 112, 93-100 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | highest activity at 100 mM | Wenyingzhuangia aestuarii |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Wenyingzhuangia aestuarii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 68.27% residual activity at 1 mM | Wenyingzhuangia aestuarii | |
| Hg2+ | 15.35% residual activity at 1 mM | Wenyingzhuangia aestuarii | |
| Mn2+ | 77.66% residual activity at 1 mM | Wenyingzhuangia aestuarii | |
| SDS | 6.4% residual activity at 1 mM | Wenyingzhuangia aestuarii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00017 | - |
kappa-carrageen | at pH 8.0 and 25°C | Wenyingzhuangia aestuarii |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 133.23% activity at 1 mM | Wenyingzhuangia aestuarii | |
| Cd2+ | 117.9% activity at 1 mM | Wenyingzhuangia aestuarii | |
| additional information | the enzyme is not significantly influenced by K+, Mg2+ and Zn2+ | Wenyingzhuangia aestuarii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| kappa-carrageen + H2O | Wenyingzhuangia aestuarii | - |
kappa-neocarratetraose + kappa-neocarrabiose | - |
? | |
| kappa-carrageen + H2O | Wenyingzhuangia aestuarii OF219 | - |
kappa-neocarratetraose + kappa-neocarrabiose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Wenyingzhuangia aestuarii | A0A223FUT7 | - |
- |
| Wenyingzhuangia aestuarii OF219 | A0A223FUT7 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HisTrap column chromatography | Wenyingzhuangia aestuarii |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, purified enzyme without any protective additives, at least 30 days, no loss of activity | Wenyingzhuangia aestuarii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| kappa-carrageen + H2O | - |
Wenyingzhuangia aestuarii | kappa-neocarratetraose + kappa-neocarrabiose | - |
? | |
| kappa-carrageen + H2O | - |
Wenyingzhuangia aestuarii OF219 | kappa-neocarratetraose + kappa-neocarrabiose | - |
? | |
| additional information | the enzyme is incapable of hydrolyzing iota-carrageenan, lambda-carrageenan and agarose | Wenyingzhuangia aestuarii | ? | - |
? | |
| additional information | the enzyme is incapable of hydrolyzing iota-carrageenan, lambda-carrageenan and agarose | Wenyingzhuangia aestuarii OF219 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 58000, recombinant enzyme, SDS-PAGE | Wenyingzhuangia aestuarii |
| ? | x * 66582, calculated from amino acid sequence | Wenyingzhuangia aestuarii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cgk16A | - |
Wenyingzhuangia aestuarii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Wenyingzhuangia aestuarii |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 30 | the enzyme shows 81% and 52% of its maximal activity at 20°C and 15°C respectively. The activity vanishes rapidly at 30°C and 40°C | Wenyingzhuangia aestuarii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
42% of residual activity is retained after 24 h at 25°C | Wenyingzhuangia aestuarii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 27.54 | - |
kappa-carrageen | at pH 8.0 and 25°C | Wenyingzhuangia aestuarii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Wenyingzhuangia aestuarii |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10.5 | the enzyme is stable for 1 h between pH 6.0-10.5 | Wenyingzhuangia aestuarii |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 165630 | - |
kappa-carrageen | at pH 8.0 and 25°C | Wenyingzhuangia aestuarii |
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