Application | Comment | Organism |
---|---|---|
additional information | the enzymatic degradation offers an alternative approach to prepare kappa-carrageenan oligosaccharides, which could be used as a powerful tool for further study on biological activity-structure relationship and thorough industrial exploitation of kappacarrageenan | Pedobacter hainanensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
x * 55000, SDS-PAGE | Pedobacter hainanensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
kappa-carrageen + H2O | Pedobacter hainanensis | - |
? | - |
? | |
kappa-carrageen + H2O | Pedobacter hainanensis 13-Q | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pedobacter hainanensis | - |
isolated from seaside soil under the stacks of red algae in Hainan province of China | - |
Pedobacter hainanensis 13-Q | - |
isolated from seaside soil under the stacks of red algae in Hainan province of China | - |
Purification (Comment) | Organism |
---|---|
native enzyme from culture supernatant by ammonium sulfate fractionation and adsorption chromatography | Pedobacter hainanensis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
700.5 | - |
purified enzyme, pH 7.0, 40°C | Pedobacter hainanensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
kappa-carrageen + H2O | - |
Pedobacter hainanensis | ? | - |
? | |
kappa-carrageen + H2O | - |
Pedobacter hainanensis 13-Q | ? | - |
? | |
kappa-carrageen + H2O | the enzyme cleaves kappa-carrageenan at the internal beta-1,4 linkage of kappa-carrageenan | Pedobacter hainanensis | carrageenan oligosaccharide | the enzyme degrades kappa-carrageenan to sulfated oligosaccharides with even-numbered degree of polymerization, of which the tetrasaccharide was the major product, identification and structural analysis by mass spectrometry, overview. Each carrageenan oligosaccharide is composed of An-G4S-type neocarrabiose units, which consisted of a 3,6-anhydro-alpha-D-galactose residue in the nonreducing end and a beta-D-galactose-4-sulfate residue in the reducing end | ? | |
kappa-carrageen + H2O | the enzyme cleaves kappa-carrageenan at the internal beta-1,4 linkage of kappa-carrageenan | Pedobacter hainanensis 13-Q | carrageenan oligosaccharide | the enzyme degrades kappa-carrageenan to sulfated oligosaccharides with even-numbered degree of polymerization, of which the tetrasaccharide was the major product, identification and structural analysis by mass spectrometry, overview. Each carrageenan oligosaccharide is composed of An-G4S-type neocarrabiose units, which consisted of a 3,6-anhydro-alpha-D-galactose residue in the nonreducing end and a beta-D-galactose-4-sulfate residue in the reducing end | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55000, SDS-PAGE | Pedobacter hainanensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Pedobacter hainanensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Pedobacter hainanensis |