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Literature summary for 3.2.1.83 extracted from

  • Kobayashi, T.; Uchimura, K.; Koide, O.; Deguchi, S.; Horikoshi, K.
    Genetic and biochemical characterization of the Pseudoalteromonas tetraodonis alkaline kappa-carrageenase (2012), Biosci. Biotechnol. Biochem., 76, 506-511.
    View publication on PubMed
Search for more literature for 3.2.1.83

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli strain DH5alpha as extracellular enzyme Pseudoalteromonas tetraodonis

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Pseudoalteromonas tetraodonis
EGTA
-
 Pseudoalteromonas tetraodonis
additional information no effect by NEM, iodoacetate, 2-phenanthroline, DTT, 2-mercaptoethanol, N-bromosuccimide, and 4-chloromercuribenzoate Pseudoalteromonas tetraodonis

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme contains an N-terminal 28-amino acid signal peptide Pseudoalteromonas tetraodonis
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Pseudoalteromonas tetraodonis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41809
-
 2 * 41809, sequence calculation Pseudoalteromonas tetraodonis
85000
-
 recombinant enzyme, gel filtration Pseudoalteromonas tetraodonis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
kappa-carrageen + H2O Pseudoalteromonas tetraodonis the enzyme cleaves in an endo-fashion ?
-
 ?
kappa-carrageen + H2O Pseudoalteromonas tetraodonis JAM-K142 the enzyme cleaves in an endo-fashion ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudoalteromonas tetraodonis E7FKF4
-
-
Pseudoalteromonas tetraodonis JAM-K142 E7FKF4
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant extracelluar enzyme 388.6fold from Escherichia coli strain DH5alpha by ammonium sulfate fractionation, dialysis, anion and cation exchange chromatography to homogeneity Pseudoalteromonas tetraodonis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.16
-
 purified recombinant enzyme, pH 6.0, 50°C Pseudoalteromonas tetraodonis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
kappa-carrageen + H2O the enzyme cleaves in an endo-fashion Pseudoalteromonas tetraodonis ?
-
 ?
kappa-carrageen + H2O the enzyme cleaves in an endo-fashion Pseudoalteromonas tetraodonis JAM-K142 ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 41809, sequence calculation Pseudoalteromonas tetraodonis

Synonyms

Synonyms Comment Organism
Cgk-K142
-
 Pseudoalteromonas tetraodonis
kappa-carrageenase-like protein
-
 Pseudoalteromonas tetraodonis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 recombinant enzyme, pH 8.0 Pseudoalteromonas tetraodonis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 no protective effect on enzyme thermal stability by Ca2+, Mg2+, agar, and NaCl Pseudoalteromonas tetraodonis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 recombinant enzyme, at 50°C Pseudoalteromonas tetraodonis
8.8
-
 recombinant enzyme, at 30°C Pseudoalteromonas tetraodonis

pI Value

Organism Comment pI Value Maximum pI Value
Pseudoalteromonas tetraodonis sequence calculation
-
 5.1