Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 63.3% residual activity at 5 mM | Pseudoalteromonas porphyrae | |
Ca2+ | 96.9% residual activity at 10 mM | Pseudoalteromonas porphyrae | |
Co2+ | 13.2% residual activity at 10 mM | Pseudoalteromonas porphyrae | |
Cu2+ | 48.3% residual activity at 10 mM | Pseudoalteromonas porphyrae | |
EDTA | 50.4% residual activity at 5 mM | Pseudoalteromonas porphyrae | |
EGTA | 51.4% residual activity at 5 mM | Pseudoalteromonas porphyrae | |
Fe3+ | 11.4% residual activity at 10 mM | Pseudoalteromonas porphyrae | |
Hg2+ | 3% residual activity at 10 mM | Pseudoalteromonas porphyrae | |
iodoacetic acid | 79.7% residual activity at 5 mM | Pseudoalteromonas porphyrae | |
K+ | 96% residual activity at 10 mM | Pseudoalteromonas porphyrae | |
Li+ | 83.1% residual activity at 10 mM | Pseudoalteromonas porphyrae | |
Mn2+ | 2.5% residual activity at 10 mM | Pseudoalteromonas porphyrae | |
Na+ | 90.5% residual activity at 10 mM | Pseudoalteromonas porphyrae | |
Ni2+ | 9.7% residual activity at 10 mM | Pseudoalteromonas porphyrae | |
phenylmethylsulfonyl fluoride | 58.6% residual activity at 5 mM | Pseudoalteromonas porphyrae | |
SDS | 22.6% residual activity at 5 mM | Pseudoalteromonas porphyrae | |
Zn2+ | 47.3% residual activity at 10 mM | Pseudoalteromonas porphyrae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 116.5% activity at 10 mM | Pseudoalteromonas porphyrae | |
Fe2+ | 105.9% activity at 10 mM | Pseudoalteromonas porphyrae | |
Mg2+ | 119% activity at 10 mM | Pseudoalteromonas porphyrae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, SDS-PAGE | Pseudoalteromonas porphyrae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudoalteromonas porphyrae | - |
- |
- |
Pseudoalteromonas porphyrae LL1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE Sepharose column chromatography and HiTrap column chromatography | Pseudoalteromonas porphyrae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
19.8 | - |
crude enzyme, at pH 8.0, 55°C | Pseudoalteromonas porphyrae |
4040 | - |
after 202.6fold purification, at pH 8.0, 55°C | Pseudoalteromonas porphyrae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
agar + H2O | 1.5% activity compared to kappa-carrageenan | Pseudoalteromonas porphyrae | ? | - |
? | |
agar + H2O | 1.5% activity compared to kappa-carrageenan | Pseudoalteromonas porphyrae LL1 | ? | - |
? | |
iota-carrageenan + H2O | 1% activity compared to kappa-carrageenan | Pseudoalteromonas porphyrae | ? | - |
? | |
iota-carrageenan + H2O | 1% activity compared to kappa-carrageenan | Pseudoalteromonas porphyrae LL1 | ? | - |
? | |
kappa-carrageenan + H2O | 100% activity | Pseudoalteromonas porphyrae | tetrasaccharides | - |
? | |
kappa-carrageenan + H2O | 100% activity | Pseudoalteromonas porphyrae LL1 | tetrasaccharides | - |
? | |
lambda-carrageenan + H2O | 39.3% activity compared to kappa-carrageenan | Pseudoalteromonas porphyrae | ? | - |
? | |
lambda-carrageenan + H2O | 39.3% activity compared to kappa-carrageenan | Pseudoalteromonas porphyrae LL1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE | Pseudoalteromonas porphyrae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Pseudoalteromonas porphyrae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
50 | the purified kappa-carrageenase is stable in the range of 0-30°C and the residual enzyme activity is still retained at 96.1% of the control after treatment at 30°C for 1 h. The enzyme is inactivated rapidly at temperatures higher than 30°C and is almost inactivated at 50°C within 1 h | Pseudoalteromonas porphyrae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pseudoalteromonas porphyrae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | the activity of the enzyme is stable from pH 6.0 to 9.0, greater than 70.0% of the residual activity is maintained after treatment at pH from 6.0 to 9.0 and 4°C for 6 h, 100% of activity is maintained after treatment at pH 8.0 and 4°C for 6 h | Pseudoalteromonas porphyrae |