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Literature summary for 3.2.1.83 extracted from

  • Liu, G.; Li, Y.; Chi, Z.; Chi, Z.
    Purification and characterization of kappa-carrageenase from the marine bacterium Pseudoalteromonas porphyrae for hydrolysis of kappa-carrageenan (2011), Process Biochem., 46, 265-271.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 63.3% residual activity at 5 mM Pseudoalteromonas porphyrae
Ca2+ 96.9% residual activity at 10 mM Pseudoalteromonas porphyrae
Co2+ 13.2% residual activity at 10 mM Pseudoalteromonas porphyrae
Cu2+ 48.3% residual activity at 10 mM Pseudoalteromonas porphyrae
EDTA 50.4% residual activity at 5 mM Pseudoalteromonas porphyrae
EGTA 51.4% residual activity at 5 mM Pseudoalteromonas porphyrae
Fe3+ 11.4% residual activity at 10 mM Pseudoalteromonas porphyrae
Hg2+ 3% residual activity at 10 mM Pseudoalteromonas porphyrae
iodoacetic acid 79.7% residual activity at 5 mM Pseudoalteromonas porphyrae
K+ 96% residual activity at 10 mM Pseudoalteromonas porphyrae
Li+ 83.1% residual activity at 10 mM Pseudoalteromonas porphyrae
Mn2+ 2.5% residual activity at 10 mM Pseudoalteromonas porphyrae
Na+ 90.5% residual activity at 10 mM Pseudoalteromonas porphyrae
Ni2+ 9.7% residual activity at 10 mM Pseudoalteromonas porphyrae
phenylmethylsulfonyl fluoride 58.6% residual activity at 5 mM Pseudoalteromonas porphyrae
SDS 22.6% residual activity at 5 mM Pseudoalteromonas porphyrae
Zn2+ 47.3% residual activity at 10 mM Pseudoalteromonas porphyrae

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 116.5% activity at 10 mM Pseudoalteromonas porphyrae
Fe2+ 105.9% activity at 10 mM Pseudoalteromonas porphyrae
Mg2+ 119% activity at 10 mM Pseudoalteromonas porphyrae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
x * 40000, SDS-PAGE Pseudoalteromonas porphyrae

Organism

Organism UniProt Comment Textmining
Pseudoalteromonas porphyrae
-
-
-
Pseudoalteromonas porphyrae LL1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE Sepharose column chromatography and HiTrap column chromatography Pseudoalteromonas porphyrae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
19.8
-
crude enzyme, at pH 8.0, 55°C Pseudoalteromonas porphyrae
4040
-
after 202.6fold purification, at pH 8.0, 55°C Pseudoalteromonas porphyrae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agar + H2O 1.5% activity compared to kappa-carrageenan Pseudoalteromonas porphyrae ?
-
?
agar + H2O 1.5% activity compared to kappa-carrageenan Pseudoalteromonas porphyrae LL1 ?
-
?
iota-carrageenan + H2O 1% activity compared to kappa-carrageenan Pseudoalteromonas porphyrae ?
-
?
iota-carrageenan + H2O 1% activity compared to kappa-carrageenan Pseudoalteromonas porphyrae LL1 ?
-
?
kappa-carrageenan + H2O 100% activity Pseudoalteromonas porphyrae tetrasaccharides
-
?
kappa-carrageenan + H2O 100% activity Pseudoalteromonas porphyrae LL1 tetrasaccharides
-
?
lambda-carrageenan + H2O 39.3% activity compared to kappa-carrageenan Pseudoalteromonas porphyrae ?
-
?
lambda-carrageenan + H2O 39.3% activity compared to kappa-carrageenan Pseudoalteromonas porphyrae LL1 ?
-
?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Pseudoalteromonas porphyrae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Pseudoalteromonas porphyrae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
50 the purified kappa-carrageenase is stable in the range of 0-30°C and the residual enzyme activity is still retained at 96.1% of the control after treatment at 30°C for 1 h. The enzyme is inactivated rapidly at temperatures higher than 30°C and is almost inactivated at 50°C within 1 h Pseudoalteromonas porphyrae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Pseudoalteromonas porphyrae

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 the activity of the enzyme is stable from pH 6.0 to 9.0, greater than 70.0% of the residual activity is maintained after treatment at pH from 6.0 to 9.0 and 4°C for 6 h, 100% of activity is maintained after treatment at pH 8.0 and 4°C for 6 h Pseudoalteromonas porphyrae