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Literature summary for 3.2.1.81 extracted from

  • An, K.; Shi, X.; Cui, F.; Cheng, J.; Liu, N.; Zhao, X.; Zhang, X.H.
    Characterization and overexpression of a glycosyl hydrolase family 16 beta-agarase YM01-1 from marine bacterium Catenovulum agarivorans YM01T (2018), Protein Expr. Purif., 143, 1-8 .
    View publication on PubMed
Search for more literature for 3.2.1.81

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Catenovulum agarivorans

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 27.39% residual activity at 10 mM Catenovulum agarivorans
Cu2+ complete inhibition at 1 mM Catenovulum agarivorans
EDTA 26.75% residual activity at 10 mM Catenovulum agarivorans
Fe3+ complete inhibition at 10 mM Catenovulum agarivorans
Mn2+ complete inhibition at 10 mM Catenovulum agarivorans
Ni2+ 90.26% residual activity at 10 mM Catenovulum agarivorans
SDS 85.76% residual activity at 10 mM Catenovulum agarivorans
Urea 85.86% residual activity at 10 mM Catenovulum agarivorans
Zn2+ 35.79% residual activity at 10 mM Catenovulum agarivorans

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 110.56% activity at 10 mM Catenovulum agarivorans
K+ 120.36% activity at 10 mM Catenovulum agarivorans
Mg2+ 208.21% activity at 10 mM Catenovulum agarivorans
Na+ 112.65% activity at 10 mM Catenovulum agarivorans

Organism

Organism UniProt Comment Textmining
Catenovulum agarivorans A0A286PT85
-
-
Catenovulum agarivorans YM01 A0A286PT85
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel-chelating affinity column chromatography Catenovulum agarivorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agarose + H2O
-
 Catenovulum agarivorans neoagarobiose
-
 ?
agarose + H2O
-
 Catenovulum agarivorans YM01 neoagarobiose
-
 ?
neoagarodecaose + H2O
-
 Catenovulum agarivorans neoagarobiose
-
 ?
neoagarodecaose + H2O
-
 Catenovulum agarivorans YM01 neoagarobiose
-
 ?
neoagarohexaose + H2O
-
 Catenovulum agarivorans neoagarobiose
-
 ?
neoagarohexaose + H2O
-
 Catenovulum agarivorans YM01 neoagarobiose
-
 ?
neoagarooctaose + H2O
-
 Catenovulum agarivorans neoagarobiose
-
 ?
neoagarooctaose + H2O
-
 Catenovulum agarivorans YM01 neoagarobiose
-
 ?
neoagarotetraose + H2O
-
 Catenovulum agarivorans neoagarobiose
-
 ?
neoagarotetraose + H2O
-
 Catenovulum agarivorans YM01 neoagarobiose
-
 ?

Subunits

Subunits Comment Organism
? x * 37700, calculated from amino acid sequence Catenovulum agarivorans

Synonyms

Synonyms Comment Organism
AGA
-
 Catenovulum agarivorans
endo-type beta-agarase
-
 Catenovulum agarivorans
YM01-1
-
 Catenovulum agarivorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Catenovulum agarivorans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 55 more than 80% of the maximum activity remain within 40-55°C Catenovulum agarivorans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 45 the enzyme is stable at temperatures below 45°C. The thermostability of the enzyme begins to decrease precipitously above 50°C, while less than 10% of maximum activity remain at 70°C Catenovulum agarivorans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2400
-
 agarose at pH 8.0 and 50°C Catenovulum agarivorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Catenovulum agarivorans

pH Range

pH Minimum pH Maximum Comment Organism
6 9 more than 50% activity between pH 6.0 and 9.0 Catenovulum agarivorans

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 more than 80% of the maximal activity is kept after preincubation at 4°C for 12 h at a pH ranging from 6.0 to 9.0 Catenovulum agarivorans

pI Value

Organism Comment pI Value Maximum pI Value
Catenovulum agarivorans calculated from amino acid sequence
-
 4.7

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2700
-
 agarose at pH 8.0 and 50°C Catenovulum agarivorans